Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:45349
Kablar B, et al., MyoD and Myf-5 differentially regulate the development of limb versus trunk skeletal muscle. Development. 1997 Dec;124(23):4729-38
|
|
Assay type:
|
Immunohistochemistry
|
|
MGI Accession ID:
|
MGI:6369412
|
|
Gene symbol:
|
Des
|
|
Gene name:
|
desmin
|
|
|
Results
|
Specimen
3A:
embryonic day 12.5
(more  )
Specimen
3A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk musculature |
Present |
Regionally restricted |
3A
|
Expression in presumptive musculature of intercostal muscles of the trunk.
|
Specimen
3B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk musculature |
Present |
Regionally restricted |
3B
|
Authors report normal distribution of expressing cells in intercostal muscles.
|
Specimen
3C:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: trunk musculature |
Weak |
Regionally restricted |
3C
|
Reduced number of expressing cells in normal location of intercostal muscle in the trunk.
|
Specimen
3D:
embryonic day 12.5
(more )
Specimen
3D:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
|
3D
|
Expression in muscle anlagen of the forelimb.
|
Specimen
3E:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Absent |
|
3E
|
No expression in muscle anlagen of forelimb.
|
Specimen
3F:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: limb |
Present |
|
3F
|
Normal number of expressing cells in forelimb buds.
|
Specimen
3G:
embryonic day 13.5
(more )
Specimen
3G:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trunk musculature |
Weak |
|
3G
|
Expression intensity decreased between 12.5 and 13.5 dpc. Authors report well-formed muscles were observed in trunk of wildtype, MyoD-/- and Myf5-/- embryos.
|
Specimen
3H:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trunk musculature |
Weak |
|
3H
|
Expression intensity decreased between 12.5 and 13.5 dpc. Authors report well-formed muscles were observed in trunk of wildtype, MyoD-/- and Myf5-/- embryos.
|
Specimen
3I:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: trunk musculature |
Weak |
|
3I
|
Expression intensity decreased between 12.5 and 13.5 dpc. Authors report well-formed muscles were observed in trunk of wildtype, MyoD-/- and Myf5-/- embryos.
|
Specimen
3J:
embryonic day 13.5
(more )
Specimen
3J:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb musculature |
Present |
|
3J
|
Authors state the distriution of expression appeared similar between wildtype and Myf5-/-.
|
Specimen
3K:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myod1tm1Jae/Myod1tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
Specimen
3L:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae
|
|
Mutant Allele(s):
|
Myf5tm1Jae/Myf5tm1Jae
|
|
Age:
|
embryonic day 13.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: limb musculature |
Present |
Regionally restricted |
3L
|
Authors state the distriution of expression appeared similar between wildtype and myf5-/- embryos.
|
|
Analysis Tools