Probe:	Hspb8_riboprobe1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	Specimens are post-fixed in 4% paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS19: heart	Strong		1A
TS19: nasal epithelium	Strong		1A
TS19: oral epithelium	Strong		1A
TS19: face	Strong	Regionally restricted	1A	Expression is in the facial epithelia.
TS19: head surface ectoderm	Strong	Regionally restricted	1A	Expression is in the facial epithelia.
TS19: forebrain	Present	Regionally restricted	1A , 1B	Expression is in the anlage of the hippocampus abutting the cortical hem and is absent in the cortical hem.
TS19: hindbrain	Present	Regionally restricted	1A	Expression is in the choroid plexus.
TS19: hindbrain ventricular layer	Present	Regionally restricted	1A , 1C	Expression is in rhombomere 4.
TS19: surface ectoderm	Present	Regionally restricted	1A	Expression is strong in the facial epithelia and weaker in the rest of the body skin.
TS19: embryo mesenchyme	Present	Regionally restricted	1A	Expression is in the mesenchyme surrounding the neural tube.
TS19: brain	Present	Regionally restricted	1B , 1A	Expression is in the choroidal stroma or mesenchymal component of the choroid plexus and is absent in the neural-derived choroidal epithelium.
TS19: telencephalon	Weak	Regionally restricted	1A	Expression is in the neocortical anlage.

Structure	Level	Pattern	Image	Note
TS19: heart	Strong		1D
TS19: nasal epithelium	Strong		1D
TS19: oral epithelium	Strong		1D
TS19: face	Strong	Regionally restricted	1D	Expression is in the facial epithelia.
TS19: head surface ectoderm	Strong	Regionally restricted	1D	Expression is in the facial epithelia.
TS19: forebrain	Present	Regionally restricted	1E , 1D	Expression is in the anlage of the hippocampus abutting the cortical hem and is absent in the cortical hem.
TS19: surface ectoderm	Present	Regionally restricted	1D	Expression is strong in the facial epithelia and weaker in the rest of the body skin.
TS19: embryo mesenchyme	Present	Regionally restricted	1D	Expression is in the mesenchyme surrounding the neural tube.
TS19: brain	Present	Regionally restricted	1D , 1E	Expression is in the choroidal stroma or mesenchymal component of the choroid plexus and is absent in the neural-derived choroidal epithelium.

Structure	Level	Pattern	Image	Note
TS21: forebrain	Present	Regionally restricted	1G	Expression is in the hippocampus.

Structure	Level	Pattern	Image	Note
TS21: 4th ventricle choroid plexus	Present		1H , 1I
TS21: lateral ventricle choroid plexus	Present		1H , 1I

Structure	Level	Pattern	Image	Note
TS23: hippocampus	Present	Regionally restricted	1L , 1M	Expression is ventral corresponding to the dentate gyrus and in the migratory stream dervied from it adjacent to the fimbriodentate junction.
TS23: dentate gyrus primordium	Present		1L , 1M
TS23: cranial ganglion	Present		1L
TS23: glossopharyngeal ganglion	Present		1L
TS23: spinal ganglion	Present		1L
TS23: brain meninges	Present	Regionally restricted	1L	Expression is in the mesenchymal tissue condensing to form the meninges.
TS23: head mesenchyme	Present	Regionally restricted	1L	Expression is in the mesenchymal tissue condensing to form the meninges.

Structure	Level	Pattern	Image	Note
TS23: trigeminal nucleus	Present	Regionally restricted	1N	Expression is in the mesencephalic trigeminal nucleus.

Structure	Level	Pattern	Image	Note
TS26: globus pallidus	Present	Scattered	2A , 2B
TS26: pallidum	Present	Scattered	2A , 2B
TS26: brain meninges	Present		2A
TS26: choroid plexus	Present		2A

Structure	Level	Pattern	Image	Note
TS26: dentate gyrus	Present	Regionally restricted	2C	Expression is in the migratory stream of the dentate gyrus occupying a subpial position and is weak in the granular cell layer.
TS26: hippocampus	Present	Regionally restricted	2C	Expression is in the migratory stream of the dentate gyrus occupying a subpial position and is weak in the granular cell layer.

Structure	Level	Pattern	Image	Note
TS28: dentate gyrus	Present	Regionally restricted	2D	Expression is in the migratory stream of the dentate gyrus occupying a subpial position.
TS28: hippocampus	Present	Regionally restricted	2D	Expression is in the migratory stream of the dentate gyrus occupying a subpial position.
TS28: anterior dorsal thalamic nucleus	Present	Uniform	2D
TS28: hippocampus granule cell layer	Weak		2D

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2E
TS28: cerebellum molecular layer	Present		2E

Structure	Level	Pattern	Image	Note
TS28: cerebellum Purkinje cell layer	Present		2F
TS28: cerebellum molecular layer	Present		2F

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong		2M , 2K
TS28: trigeminal V mesencephalic nucleus	Strong		2K
TS28: brainstem nucleus	Strong	Regionally restricted	2K	Expression is in motoneurons of the cranial motor nuclei.
TS28: accumbens nucleus	Present	Scattered	2K
TS28: brainstem reticular formation	Present	Scattered	2K
TS28: corpus striatum	Present	Scattered	2K
TS28: medial septal complex	Present	Scattered	2K
TS28: nucleus of diagonal band	Present	Scattered	2K
TS28: trigeminal V sensory nucleus	Present	Scattered	2K
TS28: vestibular nucleus	Present	Scattered	2K
TS28: amygdala	Present	Scattered	2K	There is scattered expression in the extended and central amygdala.
TS28: brainstem	Present	Scattered	2K	Expression is in the lateral lemniscus.
TS28: hippocampus granule cell layer	Present	Regionally restricted	2L , 2K	There is some basal expression.
TS28: hippocampus pyramidal cell layer	Present	Regionally restricted	2L , 2K	There is some basal expression.
TS28: dentate gyrus	Absent		2L , 2K

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong		2N
TS28: brainstem	Strong	Regionally restricted	2N	Expression is in motoneurons of the cranial motor nuclei.
TS28: accumbens nucleus	Present	Scattered	2N
TS28: brainstem reticular formation	Present	Scattered	2N
TS28: medial septal complex	Present	Scattered	2N
TS28: nucleus of diagonal band	Present	Scattered	2N
TS28: pontine nucleus	Present	Scattered	2N
TS28: trigeminal V sensory nucleus	Present	Scattered	2N
TS28: vestibular nucleus	Present	Scattered	2N
TS28: amygdala	Present	Scattered	2N	There is scattered expression in the extended and central amygdala.
TS28: corpus striatum	Present	Scattered	2P , 2N

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong		2O , 2Q
TS28: brainstem nucleus	Strong	Regionally restricted	2O , 2Q	Expression is in motoneurons of the cranial motor nuclei.
TS28: locus coeruleus	Weak	Uniform	2Q , 2O

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong	Single cells	3A , 3K	Expression is weak and homogeneous throughout the layer.
TS28: trigeminal V mesencephalic nucleus	Strong		3A
TS28: brainstem nucleus	Strong	Regionally restricted	3A	Expression is in motoneurons of the cranial motor nuclei.
TS28: brainstem reticular formation	Present	Single cells	3A
TS28: cochlear nucleus	Present	Single cells	3A
TS28: cuneate nucleus	Present	Single cells	3A
TS28: lateral medullary reticular formation	Present	Single cells	3A
TS28: lateral reticular nucleus	Present	Single cells	3A
TS28: medullary reticular formation	Present	Single cells	3A
TS28: reticular tegmental nucleus	Present	Single cells	3A
TS28: solitary tract nucleus	Present	Single cells	3A
TS28: trigeminal V principal sensory nucleus	Present	Single cells	3A
TS28: trigeminal V spinal sensory nucleus	Present	Single cells	3A
TS28: vestibular nucleus	Present	Single cells	3A
TS28: cerebellum molecular layer	Absent		3A , 3K
TS28: cerebellum white matter	Absent		3A , 3K

Structure	Level	Pattern	Image	Note
TS28: cerebellum internal granule cell layer	Strong	Single cells	3B	Expression is weak and homogeneous throughout the layer.
TS28: trigeminal V mesencephalic nucleus	Strong		3B
TS28: brainstem nucleus	Strong	Regionally restricted	3B	Expression is in motoneurons of the cranial motor nuclei.
TS28: abducens nucleus	Present	Single cells	3B
TS28: gigantocellular reticular nucleus	Present	Single cells	3B
TS28: raphe nucleus	Present	Single cells	3B
TS28: locus coeruleus	Weak	Uniform	3B
TS28: cerebellum molecular layer	Absent		3B
TS28: cerebellum white matter	Absent		3B

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong		3C
TS28: brainstem nucleus	Strong	Regionally restricted	3C	Expression is in motoneurons of the cranial motor nuclei.
TS28: pontine nucleus	Present	Single cells	3C
TS28: trigeminal V spinal sensory nucleus	Present	Single cells	3C
TS28: locus coeruleus	Weak	Uniform	3C

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong		3D
TS28: brainstem nucleus	Strong	Regionally restricted	3D	Expression is in motoneurons of the cranial motor nuclei.
TS28: laterodorsal tegmental nucleus	Present	Single cells	3D
TS28: locus coeruleus	Present	Single cells	3D
TS28: pontine nucleus	Present	Single cells	3D
TS28: trigeminal V principal sensory nucleus	Present	Single cells	3D

Structure	Level	Pattern	Image	Note
TS28: brainstem nucleus	Present	Regionally restricted	3E	Expression is in motoneurons of the cranial motor nuclei.
TS28: edinger-westphal nucleus	Present	Single cells	3E
TS28: interpeduncular nucleus	Present	Single cells	3E
TS28: midbrain tegmentum	Present	Single cells	3E
TS28: pontine nucleus	Present	Single cells	3E
TS28: red nucleus	Present	Single cells	3E
TS28: reticular tegmental nucleus	Present	Single cells	3E

Structure	Level	Pattern	Image	Note
TS28: lateral mammillary nucleus	Present		3F

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		3G

Structure	Level	Pattern	Image	Note
TS28: medial habenular nucleus	Weak		3H

Structure	Level	Pattern	Image	Note
TS28: corpus striatum	Present		3I
TS28: globus pallidus	Present		3I
TS28: medial septal nucleus	Present		3I
TS28: nucleus of diagonal band	Present		3I
TS28: amygdala	Present	Regionally restricted	3I	Expression is in the extended and central amygdala.
TS28: pallidum	Present	Regionally restricted	3I	Expression is in the ventral pallidum.
TS28: anterior dorsal thalamic nucleus	Moderate	Uniform	3I

Structure	Level	Pattern	Image	Note
TS28: globus pallidus	Present		3J
TS28: medial septal nucleus	Present		3J
TS28: nucleus of diagonal band	Present		3J
TS28: amygdala	Present	Regionally restricted	3J	Expression is in the extended and central amygdala.
TS28: pallidum	Present	Regionally restricted	3J	Expression is in the ventral pallidum.
TS28: corpus striatum	Present	Regionally restricted	3M , 3J

Structure	Level	Pattern	Image	Note
TS28: trigeminal V mesencephalic nucleus	Strong	Single cells	3L
TS28: locus coeruleus	Present	Single cells	3L

Structure	Level	Pattern	Image	Note
TS28: brainstem nucleus	Present	Regionally restricted	3O , 3Q , 3P , 3R	Expression is in motoneurons of the cranial motor nuclei.
TS28: corpus striatum	Present	Single cells	3O , 3P
TS28: accumbens nucleus	Present	Single cells	3O , 3Q
TS28: trigeminal V principal sensory nucleus	Present	Single cells	3R , 3O

Structure	Level	Pattern	Image	Note
TS28: anterior dorsal thalamic nucleus	Present	Single cells	3T , 3S