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Gene Expression Data
Assay Details
Assay
Reference: J:276416 Zhuang M, et al., The m6A reader YTHDF1 regulates axon guidance through translational control of Robo3.1 expression. Nucleic Acids Res. 2019 May 21;47(9):4765-4777
Assay type: RT-PCR
MGI Accession ID: MGI:6361559
Gene symbol: Robo3
Gene name: roundabout guidance receptor 3
Probe: Robo3.1-pA, Robo3.1-pB
Probe preparation: labelled with SYBR green
Assay notes: This assay is a quantitative RT-PCR. Gapdh was used as the standard. Anti-N6-Methyladenosine immunoprecipitation was performed before RT-PCR.
Results
Image: S6A ©

 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified (a) Amount Genetic BackgroundMutant Allele(s)Sex
E10.5 E10.5 TS17: future spinal cord Present Not Specified; total RNA Not Specified Not Specified
E11.5 E11.5 TS19: future spinal cord Present Not Specified; total RNA Not Specified Not Specified
E12.5 E12.5 TS20: spinal cord Present Not Specified; total RNA Not Specified Not Specified
Notes:
(a) Dramatic decreases of N6-Methyladenosine levels from E10.5, E11.5 to E12.5.

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory