Probe:	Sema3F probe6
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS19: lung epithelium	Weak	Regionally restricted	1G	Weak expression in distal portion of epithelium. Higher expression in distal end of bronchial trees than in lobar bronchi.
TS19: lung mesenchyme	Weak	Regionally restricted	1G	Weak expression in surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Weak	Regionally restricted	1H	Weak expression in distal portion of epithelium. Higher expression in distal end of bronchial trees than in lobar bronchi.
TS21: lung mesenchyme	Weak	Regionally restricted	1H	Weak expression in surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS21: lung epithelium	Weak	Regionally restricted	1I	Weak expression in distal portion of epithelium. Higher expression in distal end of bronchial trees than in lobar bronchi.
TS21: lung mesenchyme	Weak	Regionally restricted	1I	Weak expression in surrounding mesenchyme.

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Strong	Regionally restricted	2E	Abundunt expression in epithelial lining cells of terminal buds.
TS23: lung mesenchyme	Weak	Diffuse	2E

Structure	Level	Pattern	Image	Note
TS23: lung epithelium	Strong	Regionally restricted	2F	Abundunt expression in epithelial lining cells of terminal buds.
TS23: lung mesenchyme	Weak	Diffuse	2F

Structure	Level	Pattern	Image	Note
TS25: bronchiole epithelium	Present		3E	Mild to moderate expression in epithelium of lobar bronchi and bronchioles.
TS25: lobar bronchus epithelium	Present		3E	Mild to moderate expression in epithelium of lobar bronchi and bronchioles.
TS25: lung mesenchyme	Weak	Diffuse	3E

Structure	Level	Pattern	Image	Note
TS25: bronchiole epithelium	Present		3F	Mild to moderate expression in epithelium of lobar bronchi and bronchioles.
TS25: lobar bronchus epithelium	Present		3F	Mild to moderate expression in epithelium of lobar bronchi and bronchioles.
TS25: lung mesenchyme	Weak	Diffuse	3F