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Gene Expression Data
Assay Details
Assay
Reference: J:108721 Nishimura W, et al., A switch from MafB to MafA expression accompanies differentiation to pancreatic beta-cells. Dev Biol. 2006 May 15;293(2):526-39
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6361002
Gene symbol: Mafa
Gene name: MAF bZIP transcription factor A
Antibody: anti-MafA
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to Alexa Fluor 488
Results
Specimen 1C: postnatal adult (more )
Note: Double labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1C Expression in insulin-expressing beta cells.
Specimen 1D: postnatal adult (more )
Note: Double labeled: green - Mafa; red - glucagon (Gcg; assay MGI:6361016).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 1D
Specimen 4B: embryonic day 10.5 (more )
Note: Triple labeled: green - Mafa; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS17: pancreas primordium Absent 4B
Specimen 4E: embryonic day 11.5 (more )
Note: Triple labeled: green - Mafa; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS19: pancreas primordium Absent 4E
Specimen 4G: embryonic day 12.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS20: pancreas Present Regionally restricted 4G Rare insulin-expressing cells also express Mafa.
Specimen 4I: embryonic day 13.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS21: pancreas Present Regionally restricted 4I Rare insulin-expressing cells also express Mafa.
Specimen 4J: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 4Ja , 4Jb Expression restricted to insulin-expressing beta cells.
Specimen 5B: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - glucagon (Gcg; assay MGI:6361016); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 5B Expression restricted to insulin-expressing beta cells.
Specimen 6A: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 6A
Specimen 8B: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Glut2 (Slc2a2; assay MGI:6361021); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 8B
Specimen 8D: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Glut2 (Slc2a2; assay MGI:6361021); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 8D
Specimen 9B: embryonic day 15.5 (more )
Note: Double labeled: green - Mafa; red - Ki-67 (Mki67; assay MGI:6361022).
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 9B
Specimen 9F: postnatal day 14 (more )
Note: Double labeled: green - Mafa; red - Ki-67 (Mki67; assay MGI:6361022).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9F
Specimen 9H: postnatal day 14 (more )
Note: Triple labeled: green - Mafa; red - Ki-67 (Mki67; assay MGI:6361022); blue - Ins1 (assay MGI:6361014) and Ins2 (assay MGI:6361015).
Structure Level Pattern Image Note
TS28: endocrine pancreas Present Regionally restricted 9H
Specimen 10A: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 10A Not all Pax6-expressing cells express Mafa.
Specimen 11A: embryonic day 15.5 (more )
Note: Triple labeled: green - Mafa; red - Ins1 (assay MGI:6361010) and Ins2 (assay MGI:6361013); blue - DAPI.
Structure Level Pattern Image Note
TS23: endocrine pancreas Present Regionally restricted 11A
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
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