Hsf2 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:39331 Rallu M, et al., Function and regulation of heat shock factor 2 during mouse embryogenesis. Proc Natl Acad Sci U S A. 1997 Mar 18;94(6):2392-7
Assay type:	RNA in situ
MGI Accession ID:	MGI:6360490
Gene symbol:	Hsf2
Gene name:	heat shock factor 2

Probe:	HSF2 cDNA2
Probe preparation:	Antisense labelled with digoxigenin RNA
Assay notes:	Vibratome sections.

Results

4A ©

Specimen 4A: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: embryo	Present	Regionally restricted	4A	Expression is throughout the embryo except in the trunk mesenchyme.
TS15: branchial arch	Strong		4A
TS15: future brain	Strong		4A
TS15: future midbrain	Strong		4A
TS15: future midbrain floor plate	Strong		4A
TS15: future midbrain roof plate	Strong		4A
TS15: neural tube	Strong		4A
TS15: telencephalon	Strong		4A
TS15: telencephalon floor plate	Strong		4A
TS15: telencephalon roof plate	Strong		4A
TS15: trunk mesenchyme	Absent		4A

4B ©

Specimen 4B: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong	Regionally restricted	4B	Expression is surrounding the ventricle.
TS20: midbrain ventricular layer	Strong	Regionally restricted	4B	Expression is surrounding the ventricle.
TS20: midbrain roof plate	Absent		4B

4D ©

Specimen 4D: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present	Uniform	4D	There is homogenous expression along the antero-posterior axis with stronger expression at a more posterior level in the ependymal layer.
TS20: spinal cord roof plate	Absent		4D

4E ©

Specimen 4E: embryonic day 15.5 (more

)

Structure	Level	Image	Note
TS23: brain	Present	4E
TS23: spinal cord	Present	4E
TS23: diencephalon	Weak	4E
TS23: midbrain	Weak	4E	Expression is weaker in the anterior mesencephalon.

4G ©

Specimen 4G: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: spinal cord	Weak	Diffuse	4G

6E ©

Specimen 6E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		6E

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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