Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Gt(ROSA)26Sortm1(CAG-Eos*/Calm*)Mrc
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mario R Capecchi
MGI ID:	MGI:6360469
Synonyms:	CaMPARI2, CaMPARIlsl
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm1(CAG-Eos*/Calm*)Mrc page

Germline Transmission:	Earliest citation of germline transmission: J:101977
Parent Cell Line:	G4 (ES Cell)
Strain of Origin:	(129S6/SvEvTac x C57BL/6NCrl)F1

Allele Type:    Targeted (Conditional ready, Reporter) Mutation:    Insertion   Mutation details: The targeting construct contains a CAG promoter followed by a loxP-Stop-loxP-CaMPARI2::attB-PGK-FRT-Neo-pA-attP integrated into intron 1 of the Gt(ROSA)26Sor locus. CaMPARI (Calcium Modulated Photoactivatable Ratiometric Integrator) is a photoconvertible protein construct derived from the stony coral protein Eos. CaMPARI and irreversibly photoconverts from green to red in the presence of violet light and high intracellular calcium. The CaMPARI protein construct includes a calcium binding calmodulin with a calmodulin/Ca<2+> binding peptide (M13) fused to either end of the protein making the photoconversion calcium dependent. Calcium binding affinity variants of the original CaMPARI (CaMPARI1) fluorescent protein were identified (CaMPARI1_W391F-V398L_Q142V-F198Y-C202T-L217I-N345S) and incorporated in CaMPARI2 via targeted mutagenesis of amino acids around the fluorescent protein chromophore and at the protein interface between the fluorescent protein and the calcium-sensitive domains. (J:101977)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:101977 The Jackson Laboratory, Information obtained from The Jackson Laboratory, Bar Harbor, ME. Unpublished. 2005-2017;
All:	1 reference(s)