Foxi3 RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:198146 Shirokova V, et al., Expression of Foxi3 is regulated by ectodysplasin in skin appendage placodes. Dev Dyn. 2013 Jun;242(6):593-603
Assay type:	RNA in situ
MGI Accession ID:	MGI:6358772
Gene symbol:	Foxi3
Gene name:	forkhead box I3

Probe:	Foxi3 cDNA1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

1A ©

Specimen 1A: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Strong	Regionally restricted	1A	Expressed in a thickening of the oral epithelium (dental lamina) marks the future site of the dental arch.

1B ©

Specimen 1B: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: oral epithelium	Strong	Regionally restricted	1B	Expressed in a thickening of the oral epithelium (dental lamina) marks the future site of the dental arch.

1E ©

Specimen 1E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: molar epithelium	Present	Non-Uniform	1E	Expression became somewhat asymmetric being more intensely expressed in the lingual side of the tooth bud.

1F ©

Specimen 1F: embryonic day 17.0 (more

)

Structure	Level	Pattern	Image	Note
TS25: molar enamel organ	Strong	Regionally restricted	1F	Expressed in the inner enamel epithelium, the precursors of enamel producing ameloblasts, but it was also detected, at somewhat lower levels, in the outer enamel epithelium and in stellate reticulum in both first and second molars.
TS25: inner enamel epithelium	Strong		1F
TS25: outer enamel epithelium	Present		1F
TS25: stellate reticulum	Present		1F	Expressed in both first and second molars.

2A ©

Specimen 2A: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present	Regionally restricted	2A	Expressed in the primary hair follicles that give rise to guard hairs.

2B ©

Specimen 2B: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair epidermal placode	Present		2B

2D ©

Specimen 2D: embryonic day 19.0 (more

)

Structure	Level	Pattern	Image	Note
TS26: hair epidermal placode	Present		2D	Expressed in all hair follicle types including secondary hairs, and nascent tertiary hair placodes that give rise to zig zag hairs.

3G ©

Specimen 3G: embryonic day 9.0 (more

)

Structure	Level	Pattern	Image	Note
TS14: branchial pouch	Present		3G
TS14: alimentary system	Strong	Regionally restricted	3G	Expressed around the anal opening.

3H ©

Specimen 3H: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: maxillary process	Present		3H
TS19: naso-lacrimal groove	Present		3H
TS19: eye	Strong	Regionally restricted	3H	Expressed in the dorsal- and ventral-most part of the optic cup.

3I ©

Specimen 3I: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: eye	Strong	Regionally restricted	3I	Expressed in the dorsal- and ventral-most part of the optic cup.

3J ©

Specimen 3J: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: eye anterior segment	Strong	Regionally restricted	3J	Expressed in the anterior-most parts.

3K ©

Specimen 3K: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: naso-lacrimal groove	Present		3K
TS19: maxillary process	Present	Regionally restricted	3K	Expressed in the epithelium.

3L ©

Specimen 3L: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: integumental system	Present	Regionally restricted	3L	Expressed in the region of future nail.
TS22: hand skin	Present	Regionally restricted	3L	Expressed in a circular region lining the future tactile pads.

4A ©

Specimen 4A: embryonic day 13.5; Eda^Ta/? (more

)
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Present		4A	Reduced expression compared with the control.

4B ©

Specimen 4B: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: tooth epithelium	Present		4B

4D ©

Specimen 4D: embryonic day 14.5; Eda^Ta/? (more

)
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Absent		4D

4D' ©

Specimen 4D': embryonic day 14.5; Eda^Ta/? (more

)
Note: The mutation is X-linked; the embryo could have been homozygous or hemizygous. vibratome sections

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Absent		4D'

4E ©

Specimen 4E: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		4E

4E' ©

Specimen 4E': embryonic day 14.5 (more

)
Note: vibratome sections

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		4E'

4G ©

Specimen 4G: embryonic day 12.5; Eda^Ta/? (more

)
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		4G	Reduced expression compared with the control.

4H ©

Specimen 4H: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Present		4H

4J ©

Specimen 4J: embryonic day 13.5; Eda^Ta/? (more

)
Note: the mutation is X-linked; the embryo could have been homozygous or hemizygous

Structure	Level	Pattern	Image	Note
TS21: mammary gland primordium epithelium	Absent		4J

4K ©

Specimen 4K: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: mammary gland primordium epithelium	Absent		4K

5A ©

Specimen 5A: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: oral epithelium	Present		5A

5B ©

Specimen 5B: embryonic day 14.5; Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt, Krt14^{tm1.1(cre)Wbm}/Krt14⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: oral epithelium	Present	Regionally restricted	5B	Expressed in the ectopic signaling centers, but the expression level did not differ between control and mutant embryos.

5C ©

Specimen 5C: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		5C

5D ©

Specimen 5D: embryonic day 14.5; Ctnnb1^tm1Mmt/Ctnnb1^tm1Mmt, Krt14^{tm1.1(cre)Wbm}/Krt14⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		5D	Expressed in ectopic/precociously induced hair placodes, but the expression level did not differ between control and mutant embryos.

5E ©

Specimen 5E: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: oral epithelium	Strong	Regionally restricted	5E	Expression was down-regulated in the diastema but was maintained at high levels in incisor and molar placodes.
TS20: dental placode	Strong	Widespread	5E	Expression marked the entire dental epithelium.

5F ©

Specimen 5F: embryonic day 12.5; Sostdc1^tm1Nit/Sostdc1^tm1Nit (more

)

Structure	Level	Pattern	Image	Note
TS20: dental placode	Strong		5F	Expression levels were comparable between null and control embryos.

5G ©

Specimen 5G: embryonic day 14.5 (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		5G

5H ©

Specimen 5H: embryonic day 14.5; Sostdc1^tm1Nit/Sostdc1^tm1Nit (more

)

Structure	Level	Pattern	Image	Note
TS22: hair follicle	Present		5H	Expression levels were comparable between null and control embryos.

Specimen not shown: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: mammary bud	Weak

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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