Antibody:	anti-Fra-2
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Ab	Expression is in the basal cell layer and granular cell layer and is absent in the cornified cell layer.
TS24: hair follicle	Present	Regionally restricted	4Ab	Expression is in the epithelial component.
TS24: vibrissa follicle	Present	Regionally restricted	4Ab	Expression is in the inner and outer root sheath and is absent in the hair cortex and dermal sheath.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Ac	Expression is in the basal cell layer and granular cell layer and is absent in the cornified cell layer.
TS24: hair follicle	Present	Regionally restricted	4Ac	Expression is in the epithelial component.
TS24: vibrissa follicle	Present	Regionally restricted	4Ac	Expression is in the inner and outer root sheath and is absent in the hair cortex and dermal sheath.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Ae	Expression is in the basal cell layer and granular cell layer and is absent in the cornified cell layer.
TS24: hair follicle	Present	Regionally restricted	4Ae	Expression is in the epithelial component.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Af	Expression is in the basal cell layer and granular cell layer and is absent in the cornified cell layer.
TS24: hair follicle	Present	Regionally restricted	4Af	Expression is in the epithelial component.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Bb	Expression is in the granular and cornified cell layer lining the lumen.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Bc	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach proventricular region epithelium	Present	Regionally restricted	4Bc	Expression is beneath the cornifying cells and is weak in the basal layers.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Be	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach proventricular region epithelium	Present	Regionally restricted	4Be	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach pyloric region epithelium	Strong		4Be	Strongest expression is seen in the cornifying layers lining the lumen and the basal layers have weaker expression.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Bf	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach proventricular region epithelium	Present	Regionally restricted	4Bf	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach pyloric region epithelium	Present		4Bf	Strongest expression is seen in the cornifying layers lining the lumen and the basal layers have weaker expression.

Structure	Level	Pattern	Image	Note
TS24: skin	Present		5Aa
TS24: bone	Present	Regionally restricted	5Aa	Expression is at the bony and cartilagenous side of the growth plate.

Structure	Level	Pattern	Image	Note
TS24: oral epithelium	Present		5Ab	Strongest expression is in the cornified layer.
TS24: palate epithelium	Present		5Ab
TS24: tongue epithelium	Present		5Ab
TS24: buccal pouch	Present	Regionally restricted	5Ab	Expression is in the epithelia.

Structure	Level	Pattern	Image	Note
TS24: esophagus epithelium	Present		5Ac	Strongest expression is in the cornified layer.
TS24: trachea epithelium	Present	Regionally restricted	5Ac	Expression is in pseudostratified epithelia.

Structure	Level	Pattern	Image	Note
TS24: bone	Present	Regionally restricted	5Ad	Expression is at the bony and cartilagenous side of the growth plate.

Structure	Level	Pattern	Image	Note
TS24: intestinal villus	Present	Regionally restricted	5Ae	Expression is in the villus columnar epithelia.
TS24: intestine epithelium	Present	Regionally restricted	5Ae	Expression is in the villus columnar epithelia.

Structure	Level	Pattern	Image	Note
TS24: bronchus epithelium	Present	Regionally restricted	5Af	Expression is in pseudostratified epithelia.

Structure	Level	Pattern	Image	Note
TS24: rib	Strong		5Bb	Expression is stronger in the core layer of chondrocytes than in the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: rib	Strong		5Bc	Expression is stronger in the core layer of chondrocytes than in the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: adrenal medulla	Very strong	Regionally restricted	5Be	Expression is in chromaffin tissue in nests of cells resembling sympathetic neuroblasts.

Structure	Level	Pattern	Image	Note
TS24: adrenal medulla	Very strong	Regionally restricted	5Bf	Expression is in chromaffin tissue in nests of cells resembling sympathetic neuroblasts.

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present		6e
TS23: renal pelvis	Present		6e
TS23: renal tubule	Present		6e
TS23: ureter	Present		6e
TS23: maturing glomerular tuft	Absent		6e
TS23: mesenchyme of metanephros	Absent		6e

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present		6f
TS23: renal pelvis	Present		6f
TS23: renal tubule	Present		6f
TS23: ureter	Present		6f
TS23: maturing glomerular tuft	Absent		6f
TS23: mesenchyme of metanephros	Absent		6f

Structure	Level	Pattern	Image	Note
TS24: Meckel's cartilage	Absent
TS24: dorsal root ganglion	Absent

Structure	Level	Pattern	Image	Note
TS27: epidermis basal layer	Present
TS27: epidermis granular layer	Present
TS27: epidermis cornified layer	Absent