Probe:	fra-2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Probes were reduced to an average size of 200 nucleotides by limited alkaline hydrolysis.

Structure	Level	Pattern	Image	Note
TS11: embryo	Absent
TS11: extraembryonic component	Absent

Structure	Level	Pattern	Image	Note
TS28: decidua	Present	Regionally restricted		Expression is in decidual cells surrounding the embryo.

Structure	Level	Pattern	Image	Note
TS24: clavicle	Strong		2A/B
TS24: maxilla	Strong		2A/B
TS24: rib	Strong		2A/B
TS24: skin	Strong		2A/B
TS24: vertebra	Strong		2A/B
TS24: femur	Strong	Regionally restricted	2A/B	Expression is in growth regions of the femoral head.
TS24: cerebral cortex	Present		2A/B
TS24: metanephros	Present		2A/B
TS24: small intestine	Present		2A/B
TS24: stomach pyloric region	Present		2A/B
TS24: umbilical cord	Present		2A/B

Structure	Level	Pattern	Image	Note
TS17: embryo	Absent		3A/B

Structure	Level	Pattern	Image	Note
TS28: decidua	Present		3A/B

Structure	Level	Pattern	Image	Note
TS21: adrenal gland	Present		3C/D
TS21: bone	Present		3C/D
TS21: cerebral cortex	Present		3C/D
TS21: metanephros	Present		3C/D
TS21: nervous system	Present		3C/D
TS21: epidermis	Strong		3C/D	The strongest expression is in the ventral skin covering the abdomen.
TS21: hair follicle	Strong	Regionally restricted	3C/D	Expression is in the epithelial component.
TS21: vibrissa follicle	Strong	Regionally restricted	3C/D	Expression is in the epithelial component.
TS21: nasal cavity	Strong		3C/D
TS21: oral epithelium	Strong		3C/D
TS21: rib pre-cartilage condensation	Strong		3C/D
TS21: small intestine epithelium	Strong		3C/D
TS21: stomach proventricular region epithelium	Strong		3C/D
TS21: tongue epithelium	Strong		3C/D
TS21: urothelium	Strong		3C/D
TS21: mandible	Strong		3C/D	Expression is highest at the level of the primordium of the lower and upper incisor teeth.
TS21: urothelium of ureter	Present	Regionally restricted	3C/D	Expression is in cell clusters around the tip.

Structure	Level	Pattern	Image	Note
TS24: adrenal medulla	Very strong	Regionally restricted	3E/F	Expression is in the chromaffin tissue.
TS24: bone	Present		3E/F
TS24: cerebral cortex	Present		3E/F
TS24: metanephros	Present		3E/F
TS24: nervous system	Present		3E/F
TS24: peripheral nervous system	Present		3E/F
TS24: skin	Present		3E/F
TS24: clavicle	Strong		3E/F
TS24: nasal cavity	Strong		3E/F
TS24: oral epithelium	Strong		3E/F
TS24: rib	Strong		3E/F
TS24: small intestine epithelium	Strong		3E/F
TS24: stomach proventricular region epithelium	Strong		3E/F
TS24: tongue epithelium	Strong		3E/F
TS24: urothelium	Strong		3E/F
TS24: mandible	Strong		3E/F	Expression is highest at the level of the primordium of the lower and upper incisor teeth.
TS24: urothelium of ureter	Present	Regionally restricted	3E/F	Expression is in cell clusters around the tip.

Structure	Level	Pattern	Image	Note
TS25: adrenal medulla	Very strong	Regionally restricted	3G/H	Expression is in the chromaffin tissue.
TS25: bone	Present		3G/H
TS25: cerebral cortex	Present		3G/H
TS25: limb long bone	Present		3G/H
TS25: metacarpal bone	Present		3G/H
TS25: metanephros	Present		3G/H
TS25: metatarsal bone	Present		3G/H
TS25: nervous system	Present		3G/H
TS25: peripheral nervous system	Present		3G/H
TS25: epidermis	Strong		3G/H	Expression is strong in the dorsal skin and skin covering the tail and limbs and is weak in skin covering the abdomen.
TS25: hair follicle	Strong	Regionally restricted	3G/H	Expression is in the epithelial component.
TS25: vibrissa follicle	Strong	Regionally restricted	3G/H	Expression is in the epithelial component.
TS25: clavicle	Strong		3G/H
TS25: nasal cavity	Strong		3G/H
TS25: oral epithelium	Strong		3G/H
TS25: rib	Strong		3G/H
TS25: small intestine epithelium	Strong		3G/H
TS25: stomach proventricular region epithelium	Strong		3G/H
TS25: tongue epithelium	Strong		3G/H
TS25: urothelium	Strong		3G/H
TS25: mandible	Strong		3G/H	Expression is highest at the level of the primordium of the lower and upper incisor teeth.
TS25: urothelium of ureter	Present	Regionally restricted	3G/H	Expression is in cell clusters around the tip.

Structure	Level	Pattern	Image	Note
TS27: adrenal medulla	Very strong	Regionally restricted	3I/J	Expression is in the chromaffin tissue.
TS27: adrenal gland	Present		3I/J
TS27: bone	Present		3I/J
TS27: cerebral cortex	Present		3I/J
TS27: metanephros	Present		3I/J
TS27: nervous system	Present		3I/J
TS27: peripheral nervous system	Present		3I/J
TS27: skin	Strong		3I/J	Expression is strong in the dorsal skin and skin covering the tail and limbs and is weak in skin covering the abdomen.
TS27: hair follicle	Strong	Regionally restricted	3I/J	Expression is in the epithelial component.
TS27: vibrissa follicle	Strong	Regionally restricted	3I/J	Expression is in the epithelial component.
TS27: clavicle	Strong		3I/J
TS27: nasal cavity	Strong		3I/J
TS27: oral epithelium	Strong		3I/J
TS27: rib	Strong		3I/J
TS27: small intestine epithelium	Strong		3I/J
TS27: stomach proventricular region epithelium	Strong		3I/J
TS27: tongue epithelium	Strong		3I/J
TS27: urothelium	Strong		3I/J
TS27: mandible	Strong		3I/J	Expression is highest at the level of the primordium of the lower and upper incisor teeth.
TS27: urothelium of ureter	Present	Regionally restricted	3I/J	Expression is in cell clusters around the tip.
TS27: urothelium of bladder	Present	Regionally restricted	3I/J	Expression is in the transitional epithelium.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Aa	Expression is in the basal cell layer and granular cell layer.
TS24: hair follicle	Present	Regionally restricted	4Aa	Expression is in the epithelial component.
TS24: vibrissa follicle	Present	Regionally restricted	4Aa	Expression is in the inner and outer root sheath and is absent in the hair cortex and dermal sheath.

Structure	Level	Pattern	Image	Note
TS24: epidermis	Present	Regionally restricted	4Ad	Expression is in the basal cell layer and granular cell layer.
TS24: hair follicle	Present	Regionally restricted	4Ad	Expression is in the epithelial component.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Ba	Expression is in the granular and cornified cell layer lining the lumen.

Structure	Level	Pattern	Image	Note
TS24: stomach epithelium	Present	Regionally restricted	4Bd	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach proventricular region epithelium	Present	Regionally restricted	4Bd	Expression is in the granular and cornified cell layer lining the lumen.
TS24: stomach pyloric region epithelium	Present		4Bd

Structure	Level	Pattern	Image	Note
TS24: rib	Strong		5Ba	Expression is stronger in the core layer of chondrocytes than in the perichondrium.

Structure	Level	Pattern	Image	Note
TS24: adrenal medulla	Very strong	Regionally restricted	5Bd	Expression is in the chromaffin tissue.

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present		6a/b
TS23: renal pelvis	Present		6a/b
TS23: renal tubule	Present		6a/b
TS23: ureter	Present		6a/b
TS23: maturing glomerular tuft	Absent		6a/b
TS23: mesenchyme of metanephros	Absent		6a/b

Structure	Level	Pattern	Image	Note
TS23: comma-shaped body	Present		6c/d
TS23: renal pelvis	Present		6c/d
TS23: renal tubule	Present		6c/d
TS23: ureter	Present		6c/d
TS23: maturing glomerular tuft	Absent		6c/d
TS23: mesenchyme of metanephros	Absent		6c/d

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Strong		6g/h

Structure	Level	Pattern	Image	Note
TS28: caudate nucleus	Strong		7a/b
TS28: occipital cortex	Strong		7a/b
TS28: piriform cortex	Strong		7a/b
TS28: subiculum	Strong		7a/b
TS28: caudate-putamen	Present		7a/b
TS28: hippocampus	Present		7a/b
TS28: hypothalamus	Present		7a/b
TS28: neocortex layer	Present		7a/b
TS28: cerebral cortex	Strong	Graded	7a/b	Strongest levels are in the occipital region.

Structure	Level	Pattern	Image	Note
TS28: occipital cortex	Strong		7c/d
TS28: piriform cortex	Strong		7c/d
TS28: subiculum	Strong		7c/d
TS28: hippocampus	Present		7c/d
TS28: neocortex layer	Present		7c/d

Structure	Level	Pattern	Image	Note
TS28: amygdala	Very strong		7e/f
TS28: dentate gyrus	Very strong		7e/f
TS28: caudate nucleus	Strong		7e/f
TS28: occipital cortex	Strong		7e/f
TS28: piriform cortex	Strong		7e/f
TS28: subiculum	Strong		7e/f
TS28: caudate-putamen	Present		7e/f
TS28: hippocampus	Present		7e/f
TS28: hypothalamus	Present		7e/f
TS28: neocortex layer	Present		7e/f

Structure	Level	Pattern	Image	Note
TS28: amygdala	Very strong	Regionally restricted	7g/h

Structure	Level	Pattern	Image	Note
TS20: metanephros	Present