Gene Expression Data
Assay Details
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Assay
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Reference:
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J:74069
Morris-Wiman J, et al., Temporospatial distribution of matrix metalloproteinase and tissue inhibitors of matrix metalloproteinases during murine secondary palate morphogenesis. Anat Embryol (Berl). 2000 Aug;202(2):129-41
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6342622
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Gene symbol:
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Mmp3
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Gene name:
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matrix metallopeptidase 3
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Antibody:
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anti-Mmp-3
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Detection system:
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fluorescein
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Assay notes:
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Fixation with acid alcohol.
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Results
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Specimen
2G:
embryonic day 12.0
(more  )
Specimen
2G:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 12.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: palatal shelf mesenchyme |
Present |
Widespread |
2G
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Expression in all regions of mesenchyme. Relative expression greater within nasal area.
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| TS20: dental placode |
Present |
Regionally restricted |
2G
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Expression within palatal basement membrane of medial and oral epithelium, and tooth germ.
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| TS20: oral epithelium |
Present |
Regionally restricted |
2G
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Expression within palatal basement membrane of medial and oral epithelium, and tooth germ.
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Specimen
2H:
embryonic day 13.0
(more )
Specimen
2H:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 13.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: palatal shelf mesenchyme |
Strong |
Regionally restricted |
2H
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Expression increased in all regions of palatal mesenchyme, enhanced in nasomedial and central regions. except tooth germ and adjacent lateral mesenchyme.
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| TS21: palatal shelf epithelium |
Present |
Regionally restricted |
2H
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Expression in all regions of palatal epithelial basement membrane. Increased expression within nasal and oral basement membrane.
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Specimen
2I:
embryonic day 14.0
(more )
Specimen
2I:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Strong |
Regionally restricted |
2I
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Intense expression within all regions of palatal mesenchyme. Increased expression within dorsal medial nasal, maxillary and central mesenchymal regions, including mesenchyme surrounding regressing palatal seam. Expression in ventral medial and mid-oral mesenchyme less profound than in adjacent mesenchymal regions.
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| TS22: palatal shelf epithelium |
Present |
Regionally restricted |
2I
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Expression in all regions of palatal epithelial basement membrane, associated with nasal palatal epithelium, and within cells of the palatal seam.
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Specimen
gd 14-fused not shown:
embryonic day 14.0
(more )
Specimen
gd 14-fused not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 14.0
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Age Note:
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Age of embryo at noon of plug day not specified in reference.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Other - See Notes
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: palatal shelf mesenchyme |
Present |
Uniform |
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Expression almost uniformly distributed throughout palatal mesenchyme, with only slight increases in expression associated with mesenchymal bridge and forming bone.
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Analysis Tools