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Gene Expression Data
Assay Details
Assay
Reference: J:123725 Lax S, et al., CD248/Endosialin is dynamically expressed on a subset of stromal cells during lymphoid tissue development, splenic remodeling and repair. FEBS Lett. 2007 Jul 24;581(18):3550-6
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6314780
Gene symbol: Vcam1
Gene name: vascular cell adhesion molecule 1
Antibody: anti-VCAM-1-biotin (clone 429)
Detection system: Secondary antibody coupled to rhodamine
Assay notes: The authors did not specify which Vcam1 antibody was used. Anti-VCAM-1-biotin (clone MK2) may have been used (MGI:6314781).
Results
Specimen 3A E16: embryonic day 16.0 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS24: spleen Absent 3A E16
Specimen 3A Day 2: postnatal day 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS27: spleen Present 3A Day 2
Specimen 3A 2 week: postnatal week 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: spleen Strong 3A 2 week
Specimen 3A 6 week: postnatal week 6 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: spleen red pulp Present 3A 6 week
Specimen 3B E16: embryonic day 16.0 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS24: thymus Present Regionally restricted 3B E16 Expression is in a population of epithelial stromal cells.
Specimen 3B Day 2: postnatal day 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS27: thymus Present 3B Day 2
Specimen 3B 2 week: postnatal week 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: thymus Present 3B 2 week
Specimen 3B 6 week: postnatal week 6 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: thymus Present 3B 6 week
Specimen 3C E16: embryonic day 16.0 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS24: hemolymphoid system Present Regionally restricted 3C E16 Expression is in the inner medulla of the lymph node.
Specimen 3C Day 2: postnatal day 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS27: lymph node Present 3C Day 2
Specimen 3C 2 week: postnatal week 2 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: lymph node Weak 3C 2 week
Specimen 3C 6 week: postnatal week 6 (more )
Note: Triple labeled: red - Vcam1; green - Cd248 (assay MGI:6314775); blue - ER-TR7 (reticular fibroblast marker).
Structure Level Pattern Image Note
TS28: lymph node Weak 3C 6 week
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory