Gene Expression Data
Assay Details
Assay
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Reference:
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J:199323
Keefe Davis T, et al., Stage specific requirement of Gfralpha1 in the ureteric epithelium during kidney development. Mech Dev. 2013 Sep-Oct;130(9-10):506-18
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6314764
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Gene symbol:
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Gfra1
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Gene name:
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glial cell line derived neurotrophic factor family receptor alpha 1
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Reporter:
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GFP
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Antibody:
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anti-GFP (Aves)
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Detection system:
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Secondary antibody coupled to DyLight 488
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Results
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Specimen
1H:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gfra1tm2.1Jmi/Gfra1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
1I:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gfra1tm2.1Jmi/Gfra1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS21: ureteric tip |
Strong |
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1I
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TS21: cap mesenchyme |
Weak |
Regionally restricted |
1I
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The staining in the mesenchyme is directly adjacent to and surrounding the distal ureteric bud tip.
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Specimen
1K:
embryonic day 15.5; Gfra1tm2.1Jmi/Gfra1 +
(more )
Note: Double labeled: green - Gfra1; white - Cdh1 (assay MGI:6314765).
Specimen
1K:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gfra1tm2.1Jmi/Gfra1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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Paraformaldehyde
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Note:
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Double labeled: green - Gfra1; white - Cdh1 (assay MGI:6314765).
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Specimen
1P:
embryonic day 13.5; Gfra1tm2.1Jmi/Gfra1 +
(more )
Note: Double labeled: green - Gfra1; red - Gfra1 (assay MGI:6314766).
Specimen
1P:
(close )
Genetic Background:
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Not Specified
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Mutant Allele(s):
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Gfra1tm2.1Jmi/Gfra1+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Gfra1; red - Gfra1 (assay MGI:6314766).
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