Probe:	Hlx cDNA3
Probe preparation:	Antisense RNA

Structure	Level	Pattern	Image	Note
TS28: retina blood vessel	Present	Regionally restricted	1B , 1A	Expressed in a subset of tip cells.

Structure	Level	Pattern	Image	Note
TS17: visceral mesothelium	Strong	Regionally restricted	1C	Expressed in the splanchnic mesoderm associated with the developing liver and lung but absent from the heart.
TS17: visceral pericardium	Absent		1C
TS17: liver	Present		1C
TS17: lung	Present		1C

Structure	Level	Pattern	Image	Note
TS17: cardinal vein	Absent		1E	Absent in the endothelium.
TS17: dorsal aorta	Absent		1E	Absent in the endothelium.
TS17: blood vessel endothelium	Present	Regionally restricted	1E	Absent in the endothelium of the larger vascular structures such as the cardinal vein and the dorsal aorta, but present in vessels branching off the aorta and in the perineural vascular plexus. No expression in the capillaries inside the neural epithelium.
TS17: midgut	Present	Regionally restricted	1E	Strongest expression.

Structure	Level	Pattern	Image	Note
TS17: vascular plexus	Present	Regionally restricted	1F	Expressed in the perineural vascular plexus.

Structure	Level	Pattern	Image	Note
TS17: future spinal cord vascular element	Absent		1H , 1G	No expression in the endothelial cells in the neural epithelium.

Structure	Level	Pattern	Image	Note
TS20: liver	Present			Downregulated expression.
TS20: visceral mesothelium	Strong	Regionally restricted		Remained enriched in splanchnic mesoderm.
TS20: dermis	Present
TS20: sclerotome	Present
TS20: brain roof plate	Present			strongest expression
TS20: spinal cord roof plate	Present			strongest expression
TS20: vascular plexus	Present	Regionally restricted		Expressed in a subset of smaller blood vessels in the perineural vascular plexus surrounding the neural epithelium.
TS20: central nervous system	Present			Expressed in the neural tube. But not in the capillaries inside the neural epithelium.