Mos RNase protection Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:34019 Yonezawa S, et al., Cochlear histopathology of the mutant bustling mouse, BUS/Idr. Acta Otolaryngol. 1996 May;116(3):409-16
Assay type:	RNase protection
MGI Accession ID:	MGI:6306871
Gene symbol:	Mos
Gene name:	Mos proto-oncogene, serine/threonine kinase

Probe:	c-mos probe
Probe preparation:	Antisense labelled with P32 RNA
Visualized with:	Autoradiography

Results

Image: 6

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	0.51 kb (a)	Amount	Genetic Background	Mutant Allele(s)	Sex
tRNA,=RNase	Control
E13 (b/b)	E13.5 (b)	TS21: embryo	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E14 (b/b)	E14.5 (b)	TS22: embryo	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E15 (b/b)	E15.5 (b)	TS23: brain	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E15 (+/b)	E15.5 (b)	TS23: brain	Present (c)	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23⁺	Not Specified
E17 (KYF)	E17.5 (b)	TS25: brain	Present	Not Specified; total RNA	KYF/MsIdr		Not Specified
E17 (b/b)	E17.5 (b)	TS25: brain	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E18 (b/b)	E18.5 (b)	TS26: brain	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E18 (b/b)	E18.5 (b)	TS26: brain	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
E18 (KYF)	E18.5 (b)	TS26: brain	Present	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
0 D (b/b)	P0	TS27: brain	Present (c)	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
0 D (BALB/c)	P0	TS27: brain	Present	Not Specified; total RNA	BALB/c		Not Specified
tRNA,+RNase	Control
10D (BALB/c)	P10	TS28: telencephalon	Present	Not Specified; total RNA	BALB/c		Not Specified
10D (b/b)	P10	TS28: telencephalon	Present (c)	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
2 M (BALB/c)	P m 2	TS28: telencephalon	Weak (c)	Not Specified; total RNA	BALB/c		Not Specified
2 M (b/b)	P m 2	TS28: telencephalon	Weak (c)	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
2 M (BALB/c)	P m 2	TS28: cerebellum	Weak (c)	Not Specified; total RNA	BALB/c		Not Specified
2 M (b/b	P m 2	TS28: cerebellum	Weak (c)	Not Specified; total RNA	involves: KYF/MsIdr	Cdh23^v-bus/Cdh23^v-bus	Not Specified
tRNA,-RNase	Control

Notes:
(a) Authors report expression in whole embryos on days 13 and 14 of gestation, and in brains from late gestational stages to 2 months of age, in all strains examined.
(b) Age normalized so that noon of plug day = E0.5.
(c) No significant difference in expression in the CNS at any stage between homozygotes and heterozygotes or normal controls.

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