Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:26500
Schmitt I, et al., Expression of the Huntington disease gene in rodents: cloning the rat homologue and evidence for downregulation in non-neuronal tissues during development. Hum Mol Genet. 1995 Jul;4(7):1173-82
|
Assay type:
|
RNA in situ
|
MGI Accession ID:
|
MGI:6295160
|
Gene symbol:
|
Htt
|
Gene name:
|
huntingtin
|
Probe:
|
Rhd2 probe
|
Probe preparation:
|
Antisense labelled with S35 RNA
|
Visualized with:
|
Autoradiography
|
|
Results
|
Specimen
6d:
embryonic day 12.5
(more )
Specimen
6d:
(close )
Genetic Background:
|
NMRI
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: liver |
Strong |
|
6d
|
|
Specimen
10a/b:
postnatal week 6
(more )
Specimen
10a/b:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: seminiferous tubule |
Present |
Regionally restricted |
10a/b
|
Expression restricted to specific seminiferous tubules.
|
Specimen
10d/e:
postnatal week 6
(more )
Specimen
10d/e:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: germ cell of testis |
Present |
Regionally restricted |
10d/e
|
Elevated expression restricted to distinct stages of spermatogenic differentiation.
|
TS28: spermatid |
Ambiguous |
Regionally restricted |
10d/e
|
Authors report pronounced expression is not observed in maturing spermatids in lumen of stage II-VI tubules.
|
Specimen
10f:
postnatal week 6
(more )
Specimen
10f:
(close )
Genetic Background:
|
NMRI
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spermatocyte |
Strong |
|
10f
|
Strong expression in spermatocytes forming a thick and dense layer within tubules.
|
TS28: seminiferous tubule epithelium |
Ambiguous |
Regionally restricted |
10f
|
Authors report pronounced expression is not observed in outer spermatogenic epithelial cells.
|
|