Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:266538
Backer S, et al., Trio GEF mediates RhoA activation downstream of Slit2 and coordinates telencephalic wiring. Development. 2018 Oct 2;145(19):dev153692
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6286245
|
|
Gene symbol:
|
Ebf1
|
|
Gene name:
|
early B cell factor 1
|
|
Probe:
|
Ebf1 probe6
|
|
Probe preparation:
|
Antisense labelled with digoxigenin RNA
|
|
Visualized with:
|
Alkaline phosphatase
|
|
|
Results
|
Specimen
4A:
embryonic day 12.5
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
involves: BALB/c
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4B:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * BALB/c
|
|
Mutant Allele(s):
|
Triotm1Mst/Triotm1Mst
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: corpus striatum |
Present |
|
4B
|
|
| TS20: ganglionic eminence |
Present |
Regionally restricted |
4B
|
Expressed in corridor cells. Ebf1-expressing cells formed an abnormal horizontal beak, which contrasts with the continuous appearance of the migratory stream in WT embryos.
|
Specimen
4C:
embryonic day 14.5
(more )
Specimen
4C:
(close )
|
Genetic Background:
|
involves: BALB/c
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
4D:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * BALB/c
|
|
Mutant Allele(s):
|
Triotm1Mst/Triotm1Mst
|
|
Age:
|
embryonic day 14.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
not shown:
embryonic day 18.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: BALB/c
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
Specimen
not shown:
(close )
|
Genetic Background:
|
involves: 129S4/SvJae * BALB/c
|
|
Mutant Allele(s):
|
Triotm1Mst/Triotm1Mst
|
|
Age:
|
embryonic day 18.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Cryosection
|
|
Analysis Tools