Probe:	TTF-2 cDNA
Probe preparation:	Antisense labelled with S35 RNA
Visualized with:	Autoradiography
Assay notes:	Transcripts were hydrolyzed by alkaline hydrolysis to an average length of 150 nucleotides.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		5A , 5A'

Structure	Level	Pattern	Image	Note
TS14: foregut epithelium	Present	Regionally restricted	5B , 5B'	Expression is in the anterior foregut.
TS14: stomatodeum	Present	Regionally restricted	5B , 5B'	Expression is posterior.
TS14: pharyngeal region	Present	Regionally restricted	5B' , 5B	Expression is in the pharyngeal membrane.

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present	Regionally restricted	5C' , 5C	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: foregut	Present	Regionally restricted	5C' , 5C	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: gut endoderm	Present	Regionally restricted	5C' , 5C	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: stomatodeum	Present	Regionally restricted	5C , 5C'	Expression is in the dorsal ectoderm.
TS17: nasal process ectoderm	Absent		5C , 5C'	Absent in the rostral ectoderm that lines the nasal process.
TS17: olfactory placode	Absent		5C' , 5C	Absent in the rostral ectoderm that lines the olfactory placode.

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present	Regionally restricted	5D' , 5D	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: foregut	Present	Regionally restricted	5D' , 5D	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: gut endoderm	Present	Regionally restricted	5D' , 5D	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: stomatodeum	Present	Regionally restricted	5D' , 5D	Expression is in the dorsal ectoderm.
TS17: Rathke's pouch	Present		5D' , 5D
TS17: thyroid primordium	Present		5D' , 5D
TS17: nasal process ectoderm	Absent		5D' , 5D	Absent in the rostral ectoderm that lines the nasal process.
TS17: olfactory placode	Absent		5D , 5D'	Absent in the rostral ectoderm that lines the olfactory placode.

Structure	Level	Pattern	Image	Note
TS17: stomatodeum	Present	Regionally restricted	5E , 5E'	Expression is in the dorsal ectoderm.
TS17: Rathke's pouch	Present		5E , 5E'
TS17: thyroid primordium	Present		5E , 5E'

Structure	Level	Pattern	Image	Note
TS17: branchial pouch endoderm	Present	Regionally restricted	5F , 5F'	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: foregut	Present	Regionally restricted	5F , 5F'	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.
TS17: gut endoderm	Present	Regionally restricted	5F , 5F'	Expression is along the endoderm of the foregut lining the visceral pouch of the branchial arches.

Structure	Level	Pattern	Image	Note
TS17: stomatodeum	Present	Regionally restricted	5G , 5G'	Expression is in the dorsal ectoderm.
TS17: nasal process ectoderm	Absent		5G , 5G'	Absent in the rostral ectoderm that lines the nasal process.
TS17: olfactory placode	Absent		5G' , 5G	Absent in the rostral ectoderm that lines the olfactory placode.

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		6F
TS13: thyroid primordium	Present		6F

Structure	Level	Pattern	Image	Note
TS13: foregut diverticulum endoderm	Present		6G
TS13: thyroid primordium	Present		6G

Structure	Level	Pattern	Image	Note
TS15: thyroid primordium	Present		6H' , 6H

Structure	Level	Pattern	Image	Note
TS17: thyroid primordium	Present		6I' , 6I

Structure	Level	Pattern	Image	Note
TS20: thyroid gland	Present		6J , 6J'

Structure	Level	Pattern	Image	Note
TS15: stomatodeum ectoderm	Present	Regionally restricted	7F' , 7F	Expression is in cells fated to become the adenohypophysis in the inner stomatodeum.

Structure	Level	Pattern	Image	Note
TS15: Rathke's pouch	Present		7G , 7G'

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		7H' , 7H

Structure	Level	Pattern	Image	Note
TS17: Rathke's pouch	Present		7I' , 7I

Structure	Level	Pattern	Image	Note
TS20: adenohypophysis	Absent		7J , 7J'

Structure	Level	Pattern	Image	Note
TS21: thyroid gland	Present		8C' , 8C

Structure	Level	Pattern	Image	Note
TS23: thyroid gland	Absent		8D' , 8D