Antibody:	anti-fibronectin
Detection system:	Secondary antibody coupled to fluorescein

Structure	Level	Pattern	Image	Note
TS3: 8-cell stage embryo	Absent		1a/b

Structure	Level	Pattern	Image	Note
TS4: compacted morula	Absent		2a/b

Structure	Level	Pattern	Image	Note
TS6: embryo	Weak	Regionally restricted	3a/b	Expression is around the cells adjacent to the blastcoel cavity between the cells of the inner cell mass.
TS6: trophectoderm	Weak	Regionally restricted	3a/b	Expression is on the blastocel side of the trophectoderm.

Structure	Level	Pattern	Image	Note
TS28: basement membrane of epithelium	Strong	Regionally restricted	3a/b	Expression is in the basement membrane beneath the uterine epithelium.
TS28: endometrium epithelium	Strong	Regionally restricted	3a/b	Expression is in the basement membrane beneath the uterine epithelium.
TS28: uterus stroma	Strong	Regionally restricted	3a/b	Expression is reticular. It is seen as a network in the mucosal stroma.

Structure	Level	Pattern	Image	Note
TS8: embryo	Weak	Regionally restricted	4a/b	Expression is as a band between ectoderm and endoderm and is absent intracellularly and is absent in ectoderm.
TS8: trophectoderm	Present	Regionally restricted	4a/b	Expression is along the inner aspect surrounding the yolk cavity.
TS8: extraembryonic ectoderm	Absent		4a/b

Structure	Level	Pattern	Image	Note
TS28: uterus stroma	Strong	Regionally restricted	4a/b	Expression is reticular. It is seen as a network in the mucosal stroma.

Structure	Level	Pattern	Image	Note
TS10: embryo	Strong	Regionally restricted	5d , 5a/b , 5c	Expression is as a zone between ectoderm and endoderm and as a band separating the mesoderm from the endoderm and ectoderm.
TS10: extraembryonic component	Strong	Regionally restricted	5d , 5a/b , 5c	Expression is as a zone between ectoderm and endoderm and as a band separating the mesoderm from the endoderm and ectoderm.
TS10: Reichert's membrane	Strong		5c , 5d , 5a/b
TS10: parietal endoderm	Present	Regionally restricted	5c , 5a/b , 5d	Expression is in a discontinuous layer at the inner surface of Reichert's membrane.
TS10: embryo mesoderm	Weak		5a/b , 5c
TS10: trophoblast giant cell	Strong		5a/b
TS10: primitive endoderm	Present	Regionally restricted	5a/b	There is granular expression is in the apical region.
TS10: ectoplacental cone	Absent		5d , 5a/b
TS10: embryo ectoderm	Absent		5d , 5a/b
TS10: extraembryonic ectoderm	Absent		5d , 5a/b

Structure	Level	Pattern	Image	Note
TS28: uterus stroma	Present	Regionally restricted	5c , 5a/b	Expression is reticular. It is seen as a network in the peripheral mucosal stroma.

Structure	Level	Pattern	Image	Note
TS12: extraembryonic mesenchyme	Present		6a/b
TS12: amnion	Present	Regionally restricted	6d , 6c , 6a/b	Expression is between the mesoderm and ectoderm.
TS12: chorion	Present	Regionally restricted	6d , 6c , 6a/b	Expression is between the mesoderm and ectoderm.
TS12: extraembryonic component	Present	Regionally restricted	6d , 6c , 6a/b	Expression is between the mesoderm and ectoderm.
TS12: embryo mesoderm	Present	Regionally restricted	6c , 6a/b , 6d	Expression is on the ectodermal and endodermal sides of the expanded mesoderm area.
TS12: trophectoderm	Strong		6a/b , 6c
TS12: embryo ectoderm	Absent		6c , 6a/b
TS12: extraembryonic ectoderm	Absent		6c , 6a/b

Structure	Level	Pattern	Image	Note
TS28: uterus stroma	Absent		6a/b	The pattern of staining changes with increasing gestational age coinciding with decidua formation.

Structure	Level	Pattern	Image	Note
TS12: amnion	Present	Regionally restricted	7a/b	Expression is between the mesoderm and ectoderm.
TS12: chorion	Present	Regionally restricted	7a/b	Expression is between the mesoderm and ectoderm.
TS12: allantois	Present	Patchy	7a/b	Expression is between the cells.
TS12: embryo ectoderm	Absent		7a/b
TS12: extraembryonic ectoderm	Absent		7a/b