Sema3a RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:95356 Kettunen P, et al., Coordination of trigeminal axon navigation and patterning with tooth organ formation: epithelial-mesenchymal interactions, and epithelial Wnt4 and Tgf{beta}1 regulate semaphorin 3a expression in the dental mesenchyme. Development. 2005 Jan;132(2):323-334
Assay type:	RNA in situ
MGI Accession ID:	MGI:6270408
Gene symbol:	Sema3a
Gene name:	sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A

Probe:	Sema3a cDNA6
Probe preparation:	Antisense labelled with S35 RNA

Results

1A1 ©

Specimen 1A1: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: lower jaw primordium	Present	Regionally restricted	1A1	Expression is in the presumptive dental mesenchyme between the thickened dental epithelium and buccal nerve.

1A2 ©

Specimen 1A2: embryonic day 12.5 (more

)
Note: Early bud stage of tooth development.

Structure	Level	Pattern	Image	Note
TS20: lower jaw primordium	Present	Regionally restricted	1A2	Expression is in the condensing dental and deep jaw mesenchyme adjacent to the trigeminal nerve branch.

1A3 ©

Specimen 1A3: embryonic day 13.5 (more

)
Note: Bud stage of tooth development.

Structure	Level	Pattern	Image	Note
TS21: lower jaw molar mesenchyme	Present	Regionally restricted	1A3	Expression is in the condensed dental mesenchyme under which the molar nerve had diverged into the buccal and shorter lingual branches.
TS21: tooth bud	Present	Regionally restricted	1A3	Expression is in the condensed dental mesenchyme under which the molar nerve had diverged into the buccal and shorter lingual branches.

1A4 ©

Specimen 1A4: embryonic day 14.5 (more

)
Note: Cap stage of tooth development.

Structure	Level	Pattern	Image	Note
TS22: lower jaw molar dental follicle	Present		1A4
TS22: lower jaw mesenchyme	Present	Regionally restricted	1A4	Expression is in the alveolar bone.

1A5 ©

Specimen 1A5: embryonic day 18.5 (more

)
Note: Bell stage of tooth development.

Structure	Level	Pattern	Image	Note
TS26: outer enamel epithelium	Present		1A5
TS26: lower jaw mesenchyme	Present	Regionally restricted	1A5	Expression is in the alveolar bone.
TS26: lower jaw molar dental papilla	Present	Regionally restricted	1A5	Expression is in the base of the dental papilla mesenchyme between the epithelial cervical loops.
TS26: bell stage tooth	Present	Regionally restricted	1A5

3A1/A2 ©

Specimen 3A1/A2: postnatal day 1 (more

)

Structure	Level	Pattern	Image	Note
TS27: lower jaw molar dental papilla	Present	Regionally restricted	3A1/A2	Expression is in the dental papilla around the secondary apical foramina.
TS27: molar dental pulp	Present	Regionally restricted	3A1/A2	Expression is in the pulp floor.
TS27: lower jaw molar mesenchyme	Present	Regionally restricted	3A1/A2	Expression is in cells adjacent to epithelial cervical loops.

3C1/C2 ©

Specimen 3C1/C2: postnatal day 4 (more

)

Structure	Level	Pattern	Image	Note
TS28: lower jaw molar mesenchyme	Present	Regionally restricted	3C1/C2	Expression is in preodontoblasts next to the epithelial cells of the pulp floor and next to the epithelial root sheaths.
TS28: molar dental pulp	Present	Regionally restricted	3C1/C2	Expression is in preodontoblasts next to the epithelial cells of the pulp floor and next to the epithelial root sheaths.

5A1/A2 ©

Specimen 5A1/A2: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: oral region	Present	Regionally restricted	5A1/A2	Expression is in the mesenchyme.
TS17: 1st branchial arch mandibular component mesenchyme	Present		5A1/A2
TS17: 1st branchial arch maxillary component mesenchyme	Present		5A1/A2

5E1/E2 ©

Specimen 5E1/E2: embryonic day 11.5 (more

)

Structure	Level	Pattern	Image	Note
TS19: lower jaw primordium	Present	Regionally restricted	5E1/E2	Expression is in the mesenchyme.

5I1/I2 ©

Specimen 5I1/I2: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: lower jaw primordium	Present	Regionally restricted	5I1/I2	Expression is in the mesenchyme.

Specimen not shown: embryonic day 16.5 (more

)
Note: Bell stage of tooth development.

Structure	Level	Pattern	Note
TS24: outer enamel epithelium	Present
TS24: lower jaw mesenchyme	Present	Regionally restricted	Expression is in the alveolar bone.
TS24: lower jaw molar dental papilla	Present	Regionally restricted	Expression is in the base of the dental papilla mesenchyme between the epithelial cervical loops.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/18/2025 MGI 6.24