Gene Expression Data
Assay Details
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Assay
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Reference:
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J:167064
L'honore A, et al., Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development. 2010 Nov;137(22):3847-56
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6260203
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Gene symbol:
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Myf5
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Gene name:
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myogenic factor 5
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5+
Pitx2tm2Sac/Pitx2+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
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2A
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Authors report expression identical to Pitx2+/+ embryos.
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Specimen
2B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5+
Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: hindlimb bud |
Present |
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2B
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Authors report no significant differences in expression between homozogous and heterozygous embryos.
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Specimen
S1A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5+
Pitx2tm2Sac/Pitx2+
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
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S1A
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Authors report expression identical to Pitx2+/+ embryos.
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Specimen
S1B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 11.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: forelimb bud |
Present |
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S1B
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Authors report no significant differences in expression between homozogous and heterozygous embryos.
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Specimen
S5A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S5B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: forelimb bud |
Present |
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S5B
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Double Pitx2 and Myf5(Mrf) loss-of-function did not appear to affect the number of expressing cells.
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Specimen
S5C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S5D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: hindlimb bud |
Present |
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S5D
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Double Pitx2 and Myf5(Mrf) loss-of-function did not appear to affect the number of expressing cells.
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Specimen
S6E:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
S6F:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ
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Mutant Allele(s):
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Myf5tm2Pas/Myf5tm2Pas
Pitx2tm2Sac/Pitx2tm2Sac
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: myotome |
Present |
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S6F
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Authors report unaltered expression in myotome of double mutants.
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Analysis Tools
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