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Gene Expression Data
Assay Details
Assay
Reference: J:167064 L'honore A, et al., Pitx2 defines alternate pathways acting through MyoD during limb and somitic myogenesis. Development. 2010 Nov;137(22):3847-56
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6260194
Gene symbol: Myod1
Gene name: myogenic differentiation 1
Antibody: anti-MyoD 5.8A
Detection system: Alexa Fluor 555
Results
Specimen 1H/I: embryonic day 11.0; Myf5tm2Pas/Myf5+ (more )
Note: Double labeled: red - Myod1; green - Pitx2 (assay MGI:6260188).
Structure Level Pattern Image Note
TS18: forelimb bud Present 1H , 1I
Specimen 3A/C: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: hindlimb bud Present 3A , 3C
Specimen 3D/F: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: hindlimb bud Present 3D , 3F Authors report almost no co-labeling with Myf5, compared with control littermates.
Specimen 5I: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5I
Specimen 5J: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: forelimb bud Present 5J
Specimen 5K: embryonic day 11.5; Myf5tm2Pas/Myf5+, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: forelimb bud Weak 5K Authors state the presence of one active Myf5 allele prevented loss of expression in about 60% of cells.
Specimen 5L: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Note: Double labeled: red - Myod1; green - Myf5 reporter (assay MGI:6260202).
Structure Level Pattern Image Note
TS19: forelimb bud Absent 5L Absence of Myf5 and Pitx2 resulted in complete loss of expression.
Specimen 6E: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2+ (more )
Structure Level Pattern Image Note
TS19: myotome Present 6E
Specimen 6G: embryonic day 11.5; Myf5tm2Pas/Myf5tm2Pas, Pitx2tm2Sac/Pitx2tm2Sac (more )
Structure Level Pattern Image Note
TS19: myotome Present Regionally restricted 6G Expression in small patch of cells in most epaxial part of myotome.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/14/2024
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