Gene Expression Data
Assay Details
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Assay
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Reference:
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J:198109
Oeschger FM, et al., Gene expression analysis of the embryonic subplate. Cereb Cortex. 2012 Jun;22(6):1343-59
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6258918
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Gene symbol:
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Rcan2
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Gene name:
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regulator of calcineurin 2
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Antibody:
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Anti-Rcan2
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Detection system:
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Secondary antibody coupled to Alexa Fluor 568
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Results
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Specimen
6A:
embryonic day 15.5; Gad1tm1.1Tama/Gad1 +
(more  )
Note: Double labeled: red - Rcan2; green - Gad1 (assay MGI:6258913).
Specimen
6A:
(close  )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Rcan2; green - Gad1 (assay MGI:6258913).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
6Aa'
,
6Aa'''
,
6Aa
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Expression was detected in horizontally orientated cells at the outer edge of the marginal zone. This was a distinct population from the Gad1-labeled cells localized at the inner edge of the marginal zone.
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| TS23: cortical subplate |
Absent |
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6Aa'
,
6Aa'''
,
6Aa
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Specimen
6D:
postnatal day 8; Gad1tm1.1Tama/Gad1 +
(more )
Note: Double labeled: red - Rcan2; green - Gad1 (assay MGI:6258913).
Specimen
6D:
(close )
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Genetic Background:
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involves: C57BL/6NCrlj * CBA/JNCrlj
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Mutant Allele(s):
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Gad1tm1.1Tama/Gad1+
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Age:
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postnatal day 8
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Rcan2; green - Gad1 (assay MGI:6258913).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS28: cerebral cortex |
Strong |
Widespread |
6Dd'
,
6Dd
,
6Dd'''
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Most of the Rcan2-positive cells outside of the subplate coexpress Gad1.
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| TS28: cortical subplate |
Moderate |
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6Dd
,
6Dd'
,
6Dd'''
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Only few Rcan2-positive cells in the subplate coexpress Gad1.
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| TS28: cortical layer V |
Present |
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6Dd'''
,
6Dd
,
6Dd'
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Over 80% of Rcan2-positive cells expressed GFP (Gad1).
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| TS28: cortical layer VI |
Present |
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6Dd'''
,
6Dd
,
6Dd'
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Over 80% of Rcan2-positive cells expressed GFP (Gad1).
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Analysis Tools