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Gene Expression Data
Assay Details
Assay
Reference: J:263979 Harley RJ, et al., Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain patterning during development. Dev Dyn. 2018 Mar 14;
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6241347
Gene symbol: Nrcam
Gene name: neuronal cell adhesion molecule
Antibody: anti-NrCAM antibody (ab24344)
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 1F,G: embryonic day 16.5 (more )
Note: Double labeled: green - Nrcam; blue - Tubb3 (assay MGI:6256847).
Structure Level Pattern Image Note
TS24: cochlear ganglion Present 1G , 1F
TS24: organ of Corti Present Graded 1G , 1F Expression is in the mature base and is absent in the less mature apex.
Specimen 1H,I: embryonic day 16.5 (more )
Note: Triple labeled: green - Nrcam; blue - Tubb3 (assay MGI:6256847); red - Myo6 (assay MGI:6241345).
Structure Level Pattern Image Note
TS24: cochlear ganglion Present 1I , 1H
TS24: organ of Corti Present 1I , 1H Expression is in membranes of hair and supporting cells and is weaker in the inner hair cell region.
Specimen 1J: embryonic day 16.5 (more )
Note: Triple labeled: green - Nrcam; blue - Gap43 (assay MGI:6241349); red - Syt1 (assay MGI:6241348).
Structure Level Pattern Image Note
TS24: cochlear ganglion Present 1J , 1M , 1L Expression is on the membranes of the afferent projections and the olivocochlear efferent projections.
Specimen 2A,B: postnatal day 1 (more )
Note: Triple labeled: green - Nrcam; blue - Tubb3 (assay MGI:6256847); red - Sox2 (assay MGI:6256848).
Structure Level Pattern Image Note
TS27: organ of Corti Present Regionally restricted 2B , 2A Expression is in membranes of nerve fibers projecting into the sensory domain.
Specimen 2C,D: postnatal day 8 (more )
Note: Double labeled: green - Nrcam; purple - Myo7a (assay MGI:6256849).
Structure Level Pattern Image Note
TS28: organ of Corti Present Regionally restricted 2C , 2D Expression is on the membranes between hair cells and supporting cells.
Specimen 2E,F: postnatal day 8; Nrcamtm1Gmt/Nrcamtm1Gmt (more )
Note: Double labeled: green - Nrcam; purple - Myo7a (assay MGI:6256849).
Structure Level Pattern Image Note
TS28: organ of Corti Absent 2E , 2F
Specimen 4A: embryonic day 14.5 (more )
Note: Triple labeled: green - Nrcam; blue - Tubb3 (assay MGI:6256847); red - Sox2 (assay MGI:6256848).
Structure Level Pattern Image Note
TS22: cochlear ganglion Present 4A , 4C
TS22: cochlea Present Regionally restricted 4A , 4C Expression is in the prosensory domain.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/09/2024
MGI 6.23 		The Jackson Laboratory