Tll1 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:148787 Sabirzhanova I, et al., Activation of Tolloid-like 1 gene expression by the cardiac specific homeobox gene Nkx2-5. Dev Growth Differ. 2009 May;51(4):403-10
Assay type:	RT-PCR
MGI Accession ID:	MGI:6203650
Gene symbol:	Tll1
Gene name:	tolloid-like

Probe:	Tll1-pE, Tll1-pF
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Reactions were normalized to Gapdh.

Results

Image: 6_Tll1

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	Size Not Specified	Amount	Genetic Background	Mutant Allele(s)	Sex
KO	E9.5	TS15: heart	Present (a)	0.05 µg; total RNA	involves: 129S4/SvJaeSor	Nkx2-5^tm1Siz/Nkx2-5^tm1Siz	Not Specified
HET	E9.5	TS15: heart	Present (b)	0.05 µg; total RNA	involves: 129S4/SvJaeSor	Nkx2-5^tm1Siz/Nkx2-5⁺	Not Specified
WT	E9.5	TS15: heart	Present	0.05 µg; total RNA	Not Specified		Not Specified

Notes:
(a) Knockouts had a nearly twofold decrease in expression compared with wild type.
(b) Heterozygotes displayed an intermediate level of expression compared with the homozygotes and wild type embryos.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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