Cdc42 RT-PCR Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122591 Cui XS, et al., Cdc42 is implicated in polarity during meiotic resumption and blastocyst formation in the mouse. Mol Reprod Dev. 2007 Jun;74(6):785-94
Assay type:	RT-PCR
MGI Accession ID:	MGI:6202328
Gene symbol:	Cdc42
Gene name:	cell division cycle 42

Probe:	Cdc42-pG, Cdc42-pH
Probe preparation:	labelled with SYBR green
Assay notes:	Quantitative RT-PCR. Relative expression was determined after normalization to the external reference (rabbit Globin mRNA).

Results

	Sample Information		Bands	Other Sample Information
Lane	Age	Structure	168 bp (a)	Amount	Genetic Background	Mutant Allele(s)	Sex	Note
GV	P w 5	TS28: primary oocyte	Present	Not Specified	(C57BL/6 x C3H/He)F1		Female	germinal vesicle oocyte
MII	P w 5	TS28: secondary oocyte	Present	Not Specified	(C57BL/6 x C3H/He)F1		Female	metaphase II oocyte
1C	E0.5 (b)	TS1: 1-cell stage conceptus	Present	Not Specified	involves: C3H/He * C57BL/6		Not Specified
2C	E1.0 (b)	TS2: 2-cell stage conceptus	Present	Not Specified	involves: C3H/He * C57BL/6		Not Specified
4C	E2.0 (b)	TS3: 4-cell stage conceptus	Present	Not Specified	involves: C3H/He * C57BL/6		Not Specified
MO	E3.0 (b)	TS3: morula-stage conceptus	Present	Not Specified	involves: C3H/He * C57BL/6		Not Specified
BL	E3.5 (b)	TS4: blastocyst-stage conceptus	Present	Not Specified	involves: C3H/He * C57BL/6		Not Specified

Notes:
(a) Expression was high in GV stage oocytes, sharply decreased in MII stage oocytes, but gradually increased from 4C to BL stage.
(b) Age assigned by curator based on morphological criteria supplied by authors.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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