Gene Expression Data
Assay Details
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Assay
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Reference:
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J:149227
Liu Y, et al., Abnormal development of the neuromuscular junction in Nedd4-deficient mice. Dev Biol. 2009 Jun 1;330(1):153-66
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6200608
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Gene symbol:
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Syt2
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Gene name:
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synaptotagmin II
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Results
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Specimen
3A:
embryonic day 13.5
(more  )
Specimen
3A:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: phrenic nerve |
Present |
Regionally restricted |
3A
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Authors state the secondary (intramuscular) branch of the primary phrenic nerve develops as a single bundle perpendicular to the long axis of the muscle fibers
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Specimen
3B:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS21: phrenic nerve |
Present |
Regionally restricted |
3B
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Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops.
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Specimen
3C:
embryonic day 15.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: phrenic nerve |
Present |
Regionally restricted |
3C
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Authors state stereotypic pattern of expression established at E13.5 is maintained throughout embryonic development. Ventral portion of diaphragm is fully innervated.
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Specimen
3D:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: phrenic nerve |
Present |
Regionally restricted |
3D
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Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops. Nerves fail to innervate entire diaphragm, leaving an uninnervated gap in ventreal protion of diaphragm.
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Specimen
3E:
embryonic day 18.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: phrenic nerve |
Present |
Regionally restricted |
3E
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Authors state stereotypic pattern of expression established at E13.5 is maintained throughout embryonic development. Numerous tertiary nerves extend from secondary branches and terminate within central region of muscle.
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| TS26: diaphragm |
Present |
Regionally restricted |
3E
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Expression in ingrowing sensory nerves at edge of diaphragm.
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Specimen
3F:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: phrenic nerve |
Present |
Regionally restricted |
3F
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Authors report secondary nerve branches are markedly defasciculated, often developing abnormal loops.
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Specimen
3G:
embryonic day 18.5
(more )
Specimen
3G:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: phrenic nerve |
Present |
Regionally restricted |
3G
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Ventral portion of diaphragm is fully innervated.
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Specimen
3H:
(close )
| Structure |
Level |
Pattern |
Image |
Note |
| TS26: phrenic nerve |
Present |
Regionally restricted |
3H
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Authors report secondary nerve branches are markedly defasciculated. Nerves fail to innervate entire diaphragm, leaving an uninnervated gap in ventreal protion of diaphragm.
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Specimen
5D +/+:
embryonic day 18.5
(more )
Note: Double labeled: green - Syt2 and Nefm; red - alpha-bungarotoxin.
Specimen
5D +/+:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - Syt2 and Nefm; red - alpha-bungarotoxin.
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Specimen
5D -/-:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4Gt(OST16211)Lex
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Note:
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Double labeled: green - Syt2 and Nefm; red - alpha-bungarotoxin.
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Analysis Tools