Gene Expression Data
Assay Details
Assay
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Reference:
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J:149227
Liu Y, et al., Abnormal development of the neuromuscular junction in Nedd4-deficient mice. Dev Biol. 2009 Jun 1;330(1):153-66
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Assay type:
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In situ reporter (knock in)
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MGI Accession ID:
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MGI:6200606
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Gene symbol:
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Nedd4
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Gene name:
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neural precursor cell expressed, developmentally down-regulated 4
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Reporter:
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lacZ
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Direct Detection
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Results
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Specimen
1C:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Specimen
1D:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Specimen
1E:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Present |
Regionally restricted |
1E
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No expression in ventral region of spinal cord.
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Specimen
E13.5 spinal cord not shown:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord meninges |
Present |
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Authors report that, at later stages (E13.5-E18.5), expression remained highly restricted to the surface of the spinal cord.
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Specimen
1F:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Specimen
1G:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: dorsal root ganglion |
Strong |
Regionally restricted |
1G
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Expression in dorsal root ganglion, predominantly in Schwann cells or satellite cells.
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Specimen
1H:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Present |
Regionally restricted |
1H
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No expression in motoneurons in ventral spinal cord.
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Specimen
1I:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Specimen
1J:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: spinal cord |
Strong |
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1J
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No expression in motoneurons in ventral spinal cord.
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Specimen
1K:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: diaphragm |
Strong |
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1K
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Intense expression in skeletal muscles.
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Specimen
1L:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: limb muscle |
Strong |
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1L
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Intense expression in skeletal muscles.
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Specimen
S1A:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS21: diaphragm |
Present |
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S1A
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Expression in skeletal muscles as early as E13.5.
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Specimen
S1B:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS23: diaphragm |
Present |
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S1B
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Expression in skeletal muscles.
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Specimen
S1D:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS26: phrenic nerve |
Present |
Regionally restricted |
S1D
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Spotty expression in Schwann cells on surface of phrenic nerve.
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Specimen
E11.5 brain not shown:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS19: brain ventricular layer |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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TS19: primitive meninges |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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Specimen
E13.5 brain not shown:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: brain meninges |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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TS21: brain ventricular layer |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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Specimen
E16.5 brain not shown:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
2% Paraformaldehyde
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Structure |
Level |
Pattern |
Image |
Note |
TS24: brain meninges |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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TS24: brain ventricular layer |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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Specimen
E18.5 brain not shown:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
|
Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
|
embryonic day 18.5
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Sex:
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Not Specified
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Type:
|
section
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Fixation:
|
2% Paraformaldehyde
|
Structure |
Level |
Pattern |
Image |
Note |
TS26: brain meninges |
Present |
|
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In the brain, expression primarily localized to meninges and ventricular zones.
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TS26: brain ventricular layer |
Present |
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In the brain, expression primarily localized to meninges and ventricular zones.
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Specimen
S2A:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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Specimen
S2B:
(close )
Genetic Background:
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involves: 129S5/SvEvBrd
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Mutant Allele(s):
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Nedd4Gt(OST16211)Lex/Nedd4+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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2% Paraformaldehyde
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