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Gene Expression Data
Assay Details
Assay
Reference: J:262835 Ke FFS, et al., Embryogenesis and Adult Life in the Absence of Intrinsic Apoptosis Effectors BAX, BAK, and BOK. Cell. 2018 May 17;173(5):1217-1230.e17
Assay type: Western blot
MGI Accession ID: MGI:6196560
Gene symbol: Mlkl
Gene name: mixed lineage kinase domain-like
Antibody: Anti-MLKL (phospho S345) antibody [EPR9515(2)] (ab196436)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: The antibody used recognizes the phosphorylated form of the protein.
Results Image: S3A pMLKL
 Sample Information Bands Other Sample Information
Lane AgeStructure Size Not Specified Genetic BackgroundMutant Allele(s)Sex
Lane 1 Mol. Wt. Marker Lane
Wt 1 E10.5 TS17: embryo Absent Not Specified Not Specified
Wt 2 E10.5 TS17: embryo Absent Not Specified Not Specified
DKO 1 E10.5 TS17: embryo Absent B6.129-Baxtm1Sjk Bak1tm1Thsn Bak1tm1Thsn/Bak1tm1Thsn, Baxtm1Sjk/Baxtm1SjkNot Specified
DKO 2 E10.5 TS17: embryo Absent B6.129-Baxtm1Sjk Bak1tm1Thsn Bak1tm1Thsn/Bak1tm1Thsn, Baxtm1Sjk/Baxtm1SjkNot Specified
TKO 1 E10.5 TS17: embryo Absent B6.Cg-Boktm1.1Ast Baxtm1Sjk Bak1tm1Thsn Bak1tm1Thsn/Bak1tm1Thsn, Baxtm1Sjk/Baxtm1Sjk, Boktm1.1Ast/Boktm1.1AstNot Specified
TKO 2 E10.5 TS17: embryo Absent B6.Cg-Boktm1.1Ast Baxtm1Sjk Bak1tm1Thsn Bak1tm1Thsn/Bak1tm1Thsn, Baxtm1Sjk/Baxtm1Sjk, Boktm1.1Ast/Boktm1.1AstNot Specified
C8-/-,MLKL-/- E10.5 TS17: embryo Absent involves: 129P2/OlaHsd * C57BL/6 Casp8tm1.1Raz/Casp8tm1.1Raz, Mlkltm1.2Wsa/Mlkltm1.2WsaNot Specified
Lane 9 Cultured cells: no data stored
Lane 10 Cultured cells: no data stored
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory