Gene Expression Data
Assay Details
Assay
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Reference:
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J:158229
Baragatti B, et al., EDHF function in the ductus arteriosus: evidence against involvement of epoxyeicosatrienoic acids and 12S-hydroxyeicosatetraenoic acid. Am J Physiol Heart Circ Physiol. 2009 Dec;297(6):H2161-8
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6196510
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Gene symbol:
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Cyp2j9
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Gene name:
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cytochrome P450, family 2, subfamily j, polypeptide 9
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Antibody:
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anti-CYP2J2
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Detection system:
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Secondary antibody coupled to fluorescein
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Assay notes:
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Two antibodies were described, but the one used in this assay was not detailed; anti-CYP2J9pep2 MGI:6196500 may have been used in this assay.
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Results
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Specimen
2A:
embryonic day 19.5
(more )
Specimen
2A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 19.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: ductus arteriosus |
Present |
Diffuse |
2A
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Diffuse expression encompassing intimal and medial layers.
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Specimen
2D/F:
embryonic day 19.5
(more )
Specimen
2D/F:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 19.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS26: ductus arteriosus |
Present |
Regionally restricted |
2D
,
2F
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Expression in endoplasmic reticulum.
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Specimen
S3A:
embryonic day 17.5
(more )
Specimen
S3A:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 17.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Embedding:
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Cryosection
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Structure |
Level |
Pattern |
Image |
Note |
TS25: ductus arteriosus |
Present |
Regionally restricted |
S3A
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Authors state the ductus arteriosus from a preterm mouse had a comparable pattern of expression.
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