Gene Expression Data
Assay Details
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Assay
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Reference:
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J:137690
Cooley MA, et al., Fibulin-1 is required for morphogenesis of neural crest-derived structures. Dev Biol. 2008 Jul 15;319(2):336-45
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6194592
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Gene symbol:
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Fbln1
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Gene name:
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fibulin 1
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Results
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Specimen
2C:
embryonic day 10.5
(more  )
Note: Triple labeled: green - Fbln1; blue - alpha-sarcomeric actin (Acta1 assay MGI:6194594); red - propidium iodide.
Specimen
2C:
(close  )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Fbln1; blue - alpha-sarcomeric actin (Acta1 assay MGI:6194594); red - propidium iodide.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: atrioventricular endocardial cushion |
Strong |
Regionally restricted |
2C
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Prominent expression in extracellular matrix surrounding mesenchymal cells of atrioventricular and outflow tract cushions.
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| TS17: outflow tract endocardial cushion |
Strong |
Regionally restricted |
2C
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Prominent expression in extracellular matrix surrounding mesenchymal cells of atrioventricular and outflow tract cushions.
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Specimen
2D:
(close )
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Genetic Background:
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involves: 129P2/OlaHsd * C57BL/6
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Mutant Allele(s):
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Fbln1Gt(XST011)Byg/Fbln1Gt(XST011)Byg
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Triple labeled: green - Fbln1; blue - alpha-sarcomeric actin (Acta1 assay MGI:6194594); red - propidium iodide.
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Specimen
7A:
embryonic day 9.5
(more )
Note: Double labeled: green - Fbln1; red - SMA (Acta2 assay MGI:6194595).
Specimen
7A:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fbln1; red - SMA (Acta2 assay MGI:6194595).
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Specimen
7B:
embryonic day 10.5
(more )
Note: Double labeled: green - Fbln1; red - SMA (Acta2 assay MGI:6194595).
Specimen
7B:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fbln1; red - SMA (Acta2 assay MGI:6194595).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: head mesenchyme |
Present |
Regionally restricted |
7B
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Expresssion in extracellular matrix of mesenchyme lateral to neural tube at level of pharyngeal arches.
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| TS17: branchial arch mesenchyme |
Weak |
Regionally restricted |
7B
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Relative low levels of expression in pharyngeal arch arch mesenchyme directly adjacent to aortic sac/distal outflow tract.
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| TS17: heart |
Present |
Regionally restricted |
7B
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Expression in secondary heart field region.
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| TS17: 4th arch artery |
Present |
Regionally restricted |
7B
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Expression in subendothelial extracellular matrix of 4th aortic arch artery.
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Specimen
7C:
embryonic day 9.5
(more )
Note: Double labeled: green - Fbln1; red - Pax3 (assay MGI:6194596).
Specimen
7C:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Fbln1; red - Pax3 (assay MGI:6194596).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: outflow tract |
Strong |
Regionally restricted |
7C
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Prominent expression in the extracellular matrix of the distal outflow tract, in the region of the secondary heart field.
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| TS15: branchial arch mesenchyme |
Strong |
Regionally restricted |
7C
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High levels of expression in pharyngeal arch mesenchyme underlying the neural tube.
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| TS15: branchial arch ectoderm |
Ambiguous |
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7C
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Authors report little or no expression in pharyngeal arch ectoderm or endoderm at E9.5-10.5.
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| TS15: branchial arch endoderm |
Ambiguous |
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7C
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Authors report little or no expression in pharyngeal arch ectoderm or endoderm at E9.5-10.5.
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Specimen
7D:
embryonic day 10.5
(more )
Specimen
7D:
(close )
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Genetic Background:
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involves: C57BL/6
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: outflow tract |
Strong |
Regionally restricted |
7D
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Prominent expression in the extracellular matrix of the distal outflow tract, in the region of the secondary heart field.
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| TS17: branchial arch ectoderm |
Ambiguous |
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7D
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Authors report little or no expression in pharyngeal arch ectoderm or endoderm at E9.5-10.5.
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| TS17: branchial arch endoderm |
Ambiguous |
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7D
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Authors report little or no expression in pharyngeal arch ectoderm or endoderm at E9.5-10.5.
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