Probe:	Pygo2 cDNA3
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase
Assay notes:	The authors used either this probe or Pygo2 cDNA2 (MGI:6194290) and obtained identical results.

Structure	Level	Pattern	Image	Note
TS20: embryo	Present	Regionally restricted	3A	Expression is nearly throughout the embryo.
TS20: midbrain	Strong		3A
TS20: spinal cord	Strong		3A
TS20: brain	Present		3A
TS20: intestine	Present		3A
TS20: liver	Present		3A
TS20: lung	Present		3A

Structure	Level	Pattern	Image	Note
TS20: diencephalon	Present		3C
TS20: diencephalon lamina terminalis	Present		3C
TS20: medulla oblongata	Present		3C
TS20: metencephalon	Present		3C
TS20: telencephalon	Present		3C
TS20: midbrain	Strong		3C	Includes the cerebral peduncle.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Strong		3D

Structure	Level	Pattern	Image	Note
TS20: spinal cord	Present		3E

Structure	Level	Pattern	Image	Note
TS20: bronchus epithelium	Present		3F

Structure	Level	Pattern	Image	Note
TS20: large intestine	Present	Regionally restricted	3G	Expression is in the epithelium of the future colon.

Structure	Level	Pattern	Image	Note
TS20: liver	Present	Scattered	3H

Structure	Level	Pattern	Image	Note
TS20: vibrissa	Present		4A

Structure	Level	Pattern	Image	Note
TS20: epidermis	Present	Scattered	4B

Structure	Level	Pattern	Image	Note
TS21: epidermis	Present		4C
TS21: hair follicle	Present	Regionally restricted	4C	Expression is in emerging pelage hair placodes and in the dermal condensates around the developing pelage placodes that will form the future dermal papilla.

Structure	Level	Pattern	Image	Note
TS23: hair epidermal placode	Present		4D
TS23: hair follicle dermal papilla	Present		4D
TS23: epidermis	Present		4D	Expression is in the future suprabasal layers.

Structure	Level	Pattern	Image	Note
TS24: hair epidermal placode	Present		4E
TS24: hair follicle dermal papilla	Present		4E
TS24: epidermis	Present	Regionally restricted	4E	Expression is in the future suprabasal layers.

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Widespread	4F
TS27: hair follicle	Present	Widespread	4F

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Widespread	5C , 5A
TS28: hair bulb	Present	Widespread	5C , 5A
TS28: hair follicle	Present	Widespread	5C , 5A

Structure	Level	Pattern	Image	Note
TS28: epidermis	Present	Widespread	5B
TS28: hair follicle	Present	Widespread	5B
TS28: hair follicle matrix region	Strong	Regionally restricted	5F , 5B	Expression is particularly prominent in the matrix of the mid-anagen hair follicles of the new hair cycle.
TS28: hair follicle dermal papilla	Weak		5F , 5B

Structure	Level	Pattern	Image	Note
TS28: hair follicle dermal papilla	Present		5D	Expression is in the ball-shaped dermal papilla at the base of the resting hair follicles in telogen hair follicles.
TS28: hair follicle bulge	Present	Regionally restricted	5D	Expression is in the stem cell-containing bulge that lines the club hair.

Structure	Level	Pattern	Image	Note
TS28: hair follicle matrix region	Strong		5E	Expression is particularly prominent in the matrix of the early-anagen hair follicles of the new hair cycle.
TS28: hair follicle dermal papilla	Weak		5E

Structure	Level	Pattern	Image	Note
TS21: embryo	Present	Widespread

Structure	Level	Pattern	Image	Note
TS23: embryo	Present	Widespread

Structure	Level	Pattern	Image	Note
TS24: embryo	Present	Widespread