Antibody:	anti-Scpep1
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Assay notes:	Data was summarized in Table 1.

Structure	Level	Pattern	Image	Note
TS15: dorsal aorta	Moderate			Expressed in smooth muscle cells.
TS15: brain ventricle	Moderate	Regionally restricted		Expressed in choroid plexus.
TS15: future brain	Moderate			Expressed in the neuronal fibers.
TS15: esophageal region epithelium	Moderate
TS15: primitive ventricle cardiac muscle	Moderate
TS15: atrium cardiac muscle	Trace			Very weak
TS15: ganglion	Moderate			Expressed in cell bodies and fibers.
TS15: foregut	Moderate	Regionally restricted		Mainly expressed in the epithelium of villi and crypt of Lieberkuhn.
TS15: hepatic primordium	Weak			Expressed in hepatocytes.
TS15: lung	Moderate			Expressed in type II alveolar cells and macrophages.
TS15: midgut	Moderate	Regionally restricted		Highly expressed in oxyntic glands of epithelium; are only weakly positive in surface mucous cell in the stomach.
TS15: muscle tissue	Trace			Very weak expression in skeletal muscle.

Structure	Level	Pattern	Image	Note
TS20: dorsal aorta	Weak			Expressed in smooth muscle cells.
TS20: brain	Moderate			Expressed in the neuronal fibers.
TS20: choroid plexus	Moderate
TS20: esophagus epithelium	Moderate
TS20: atrium cardiac muscle	Trace			very weak
TS20: skeletal muscle	Trace			very weak
TS20: mesonephros	Weak
TS20: ventricle cardiac muscle	Weak
TS20: ganglion	Moderate			Expressed in cell bodies and fibers.
TS20: intestine epithelium	Moderate			Mainly expressed in the epithelium of villi and crypt of Lieberkuhn.
TS20: liver	Weak			Expressed in hepatocytes.
TS20: lung	Moderate			Expressed in type II alveolar cells and macrophages.
TS20: stomach	Moderate	Regionally restricted		Highly expressed in oxyntic glands of epithelium; are only weakly positive in surface mucous cell.

Structure	Level	Pattern	Image	Note
TS23: aorta	Weak			Expressed in smooth muscle cell.
TS23: brain	Weak			Expressed in the neuronal fibers.
TS23: atrium cardiac muscle	Weak
TS23: choroid plexus	Weak
TS23: islets of Langerhans	Weak
TS23: esophagus epithelium	Moderate
TS23: mesonephros	Moderate
TS23: skeletal muscle	Trace			very weak
TS23: ventricle cardiac muscle	Trace			very weak
TS23: ganglion	Moderate			Expressed in cell bodies and fibers.
TS23: intestine epithelium	Moderate			Mainly expressed in the epithelium of villi and crypt of Lieberkuhn.
TS23: liver	Weak			Expressed in hepatocytes.
TS23: lung	Moderate			Expressed in type II alveolar cells and macrophages.
TS23: stomach	Moderate	Regionally restricted		Highly expressed in oxyntic glands of epithelium; are only weakly positive in surface mucous cell.

Structure	Cell Type	Level	Pattern	Image	Note
TS28: aorta smooth muscle		Weak
TS28: choroid plexus		Weak
TS28: brain		Weak			Expressed in the neuronal fibers.
TS28: crypt of lieberkuhn		Moderate
TS28: esophagus epithelium		Moderate
TS28: intestinal villus		Moderate
TS28: islets of Langerhans		Moderate
TS28: urothelium of bladder		Moderate
TS28: atrium cardiac muscle		Trace			very weak
TS28: renal distal tubule		Trace			very weak
TS28: skeletal muscle		Trace			very weak
TS28: ventricle cardiac muscle		Trace			very weak
TS28: ganglion		Weak			Expressed in cell bodies and fibers.
TS28: macula densa		Strong
TS28: renal proximal convoluted tubule		Strong
TS28: spermatogonium		Strong
TS28: Leydig cell	Leydig cell	Strong
TS28: Sertoli cell	Sertoli cell	Strong
TS28: liver		Strong			Expressed in hepatocytes.
TS28: alveolus epithelium		Moderate			Expressed in type II alveolar cells.
TS28: lung		Moderate			Expressed in macrophages.
TS28: lymph node		Moderate			Expressed in macrophage-like cells.
TS28: stomach glandular epithelium		Strong	Regionally restricted		Highly expressed in oxyntic glands of epithelium.
TS28: stomach mucosa		Weak			Expressed in surface mucous cells.
TS28: spleen		Strong			Expressed in macrophage-like cells.