Gene Expression Data
Assay Details
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Assay
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Reference:
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J:130823
Mitsiadis TA, et al., Deletion of the Pitx1 genomic locus affects mandibular tooth morphogenesis and expression of the Barx1 and Tbx1 genes. Dev Biol. 2008 Jan 15;313(2):887-96
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6188911
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Gene symbol:
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Pitx2
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Gene name:
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paired-like homeodomain transcription factor 2
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Results
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Specimen
2B:
postnatal newborn
(more  )
Specimen
2B:
(close  )
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Genetic Background:
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Swiss
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cervical loop |
Present |
Regionally restricted |
2B
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Authors state that ameloblast differentiation coincided with down regulation of expression. Expression in inner enamel epithelium was restricted, only in cervical loop.
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Specimen
2D:
postnatal newborn
(more )
Specimen
2D:
(close )
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Genetic Background:
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Swiss
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2F:
postnatal newborn
(more )
Specimen
2F:
(close )
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Genetic Background:
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Swiss
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Age:
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postnatal newborn
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6A:
embryonic day 18.0
(more )
Note: Double labeled: red - Pitx2; violet - Barx1 (assay MGI:6188913).
Specimen
6A:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pitx2; violet - Barx1 (assay MGI:6188913).
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Specimen
6B:
embryonic day 18.0
(more )
Note: Double labeled: red - Pitx2; violet - Barx1 (assay MGI:6188913).
Specimen
6B:
(close )
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Genetic Background:
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Swiss
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: red - Pitx2; violet - Barx1 (assay MGI:6188913).
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Specimen
6C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
6D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
7A:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: molar enamel organ |
Present |
Regionally restricted |
7A
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Authors report no alternation in expression pattern of developing molars of mutant mice. Expression in epithelium of first and second molars, equal in intensity for teeth of mandible and maxilla. Down regulation of expresion at tip of cusps coinciding with pre-ameloblast differentiation. Strong expression in epithelium of second molar anlagen.
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Specimen
7B:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: upper jaw molar enamel organ |
Present |
Regionally restricted |
7B
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Authors report no alternation in expression pattern of developing molars of mutant mice. Expression in first maxillary molar. Expression downregulated in preameloblasts.
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Specimen
7C:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: upper jaw molar enamel organ |
Present |
Regionally restricted |
7C
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Expresion in second maxillary molar. Down regulation of expression at tip of cusps coinciding with pre-ameloblast differentiation. Strong expression in epithelium of second molar anlagen.
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Specimen
7D:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * BALB/c
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Mutant Allele(s):
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Pitx1tm1Jdr/Pitx1tm1Jdr
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Age:
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embryonic day 18.0
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: lower jaw molar enamel organ |
Present |
Regionally restricted |
7D
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Expression first and second mandibular molars. Down regulation of expression at tip of cusps coinciding with pre-ameloblast differentiation. Strong expression in epithelium of second molar anlagen.
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