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Gene Expression Data
Assay Details
Assay
Reference: J:259556 Bottger P, et al., Glutamate-system defects behind psychiatric manifestations in a familial hemiplegic migraine type 2 disease-mutation mouse model. Sci Rep. 2016 Feb 25;6:22047
Assay type: Western blot
MGI Accession ID: MGI:6187878
Gene symbol: Atp1a2
Gene name: ATPase, Na+/K+ transporting, alpha 2 polypeptide
Antibody: anti-human alpha2 (07-674)
Detection system: Secondary antibody coupled to horseradish peroxidase
Assay notes: Densitometric quantification is shown in figure S2c and additional screening information and protein quantifications are shown in Supplementary Table 2.
Results
Image: S2a ©

 Sample Information Bands Other Sample Information
Lane AgeStructure 110 kDa Genetic BackgroundMutant Allele(s)Sex
+/+ E17.5 TS25: brain Present C57BL/6J Not Specified
+/- E17.5 TS25: brain Present (a) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Not Specified
-/- E17.5 TS25: brain Weak (b) involves: 129S1/Sv * C57BL/6J Atp1a2tm1.1Tmklh/Atp1a2tm1.1TmklhNot Specified
+/+ P newborn TS27: brain Present C57BL/6J Not Specified
+/- P newborn TS27: brain Present (a) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Not Specified
-/- P newborn TS27: brain Weak (c) involves: 129S1/Sv * C57BL/6J Atp1a2tm1.1Tmklh/Atp1a2tm1.1TmklhNot Specified
C5 +/+ P adult TS28: brain Present C57BL/6J Male
M Mol. Wt. Marker Lane
HC +/+ P adult TS28: hippocampus Present C57BL/6J Male
HC +/- P adult TS28: hippocampus Present (d) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Male
CRBL +/+ P adult TS28: cerebellum Present C57BL/6J Male
CRBL -/+ P adult TS28: cerebellum Present (d) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Male
CTX +/+ P adult TS28: cerebral cortex Present C57BL/6J Male
CTX +/- P adult TS28: cerebral cortex Present (d) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Male
BS +/+ P adult TS28: brainstem Present C57BL/6J Male
BS +/- P adult TS28: brainstem Present (d) B6.129S1(Cg)-Atp1a2tm1.1Tmklh Atp1a2tm1.1Tmklh/Atp1a2+Male
Notes:
(a) There is a 33-41% reduction in protein levels compared to wild-type.
(b) There is a 71% reduction in protein levels compared to wild-type.
(c) There is a 94% reduction in protein levels compared to wild-type.
(d) There is a 36-78% reduction in protein levels in the brain areas analyzed compared to wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
MGI 6.23 		The Jackson Laboratory