Gene Expression Data
Assay Details
Assay
|
Reference:
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J:253966
Sigg MA, et al., Evolutionary Proteomics Uncovers Ancient Associations of Cilia with Signaling Pathways. Dev Cell. 2017 Dec 18;43(6):744-762.e11
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Assay type:
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RNA in situ
|
MGI Accession ID:
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MGI:6156677
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Gene symbol:
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Enkur
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Gene name:
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enkurin, TRPC channel interacting protein
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Probe:
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Enkur cDNA1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
|
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Results
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Specimen
6E Enkur+/+ left:
embryonic day 8.0
(more )
Note: 2-4 somite stage embryo.
Specimen
6E Enkur+/+ left:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
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Age:
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embryonic day 8.0
|
Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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2-4 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
6E Enkur+/+ left
|
|
Specimen
6E Enkur+/+ right:
embryonic day 8.0
(more )
Note: 2-4 somite stage embryo.
Specimen
6E Enkur+/+ right:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-4 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
|
6E Enkur+/+ right
|
|
Specimen
6E Enkur-/- left:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
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Mutant Allele(s):
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Enkurtm1Hmf/Enkurtm1Hmf
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Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-4 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Absent |
|
6E Enkur-/- left
|
|
Specimen
6E Enkur-/- right:
(close )
Genetic Background:
|
involves: 129 * C57BL/6J
|
Mutant Allele(s):
|
Enkurtm1Hmf/Enkurtm1Hmf
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
2-4 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Absent |
|
6E Enkur-/- right
|
|
Specimen
6K top:
embryonic day 8.0
(more )
Note: 2-4 somite stage embryo.
Specimen
6K top:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 8.0
|
Age Note:
|
Age assigned by curator based on morphological criteria supplied by authors.
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Cryosection
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Note:
|
2-4 somite stage embryo.
|
Structure |
Level |
Pattern |
Image |
Note |
TS12: node |
Present |
Regionally restricted |
6K bottom
,
6K top
,
6K middle
|
Expression is predominantly in the pit cells and is expressed weakly or is absent in the crown cells of the node.
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|