Gene Expression Data
Assay Details
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Assay
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Reference:
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J:238219
Fabrizius A, et al., Critical re-evaluation of neuroglobin expression reveals conserved patterns among mammals. Neuroscience. 2016 Nov 19;337:339-354
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Assay type:
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RT-PCR
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MGI Accession ID:
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MGI:6156308
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Gene symbol:
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Ngb
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Gene name:
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neuroglobin
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Probe:
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Ngb-qPCR-Dev-For, Ngb-qPCR-Dev-Rev
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Probe preparation:
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labelled with SYBR green
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Assay notes:
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Two set of primers were described in the methods: MGI:6156293 and MGI:6156295. The publication didn't specify which was used in this assay. Quantitative RT-PCR. Data were normalized to histone H2a mRNA expression as housekeeping gene. The calculated Ngb/H2a ratio in embryonic stage E10 was set to 1 and the other developmental stages are shown in relation.
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Results
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Image: 3B
| E10.0
(a)
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TS16: future brain
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Present
(c)
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1 µg; total RNA |
BALB/c |
| Not Specified |
The E10 sample was used as the standard and was not explicitly displayed on the graph.
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| E15.0
(a)
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TS23: brain
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Present
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1 µg; total RNA |
BALB/c |
| Not Specified |
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| E19.0
(a)
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TS26: brain
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Present
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1 µg; total RNA |
BALB/c |
| Not Specified |
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| P1
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TS27: brain
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Present
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1 µg; total RNA |
BALB/c |
| Not Specified |
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| P adult
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TS28: brain
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Present
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1 µg; total RNA |
BALB/c |
| Not Specified |
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Notes:
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Analysis Tools
