Ccdc88c Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:251823 Takagishi M, et al., Daple Coordinates Planar Polarized Microtubule Dynamics in Ependymal Cells and Contributes to Hydrocephalus. Cell Rep. 2017 Jul 25;20(4):960-972
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6155670
Gene symbol:	Ccdc88c
Gene name:	coiled-coil domain containing 88C

Antibody:	anti-Daple
Detection system:	Secondary antibody coupled to Alexa Fluor

Results

4A +/+ ©

Specimen 4A +/+: postnatal day 21 (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	4A +/+	Authors state that fixation with methanol only results in expression in anterior cell membrane at the apical surface.

4A -/- ©

Specimen 4A -/-: postnatal day 21; Ccdc88c^tm1(KOMP)Mbp/Ccdc88c^tm1(KOMP)Mbp (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Absent		4A -/-	No expression in ependymal cells.

4B ©

4B (inset) ©

4B Daple ©

Specimen 4B: postnatal day 21 (more

)
Note: Triple labeled: green - Ccdc88c; red - Ctnnb1 (assay MGI:6155669); purple - gamma tubulin. Specimen sequentially fixed with methanol and paraformaldehyde.

Structure	Level	Pattern	Image	Note
TS28: lateral ventricle ependyma	Present	Regionally restricted	4B , 4B (inset) , 4B Daple	Authors report sequential fixation with methanol and paraformaldehyde resulted in expression near basal bodies.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 04/30/2024 MGI 6.23