Summary | Mutation origin | Mutation description | Find Mice (IMSR) | References

Symbol:	Tn(pb-CAG-cas9*,-EGFP)1Yangh
Name:	transposon insertion 1, Hui Yang
MGI ID:	MGI:6149220
Synonyms:	SPH
Gene:	Tn(pb-CAG-cas9*,-EGFP)1Yangh  Location: unknown  Genetic Position: Chr14, Syntenic

Strain of Origin:

(C57BL/6 x DBA/2J)F1

Allele Type:    Transposon induced (Conditional ready, Endonuclease, Epitope tag, Reporter) Mutation:    Transposon insertion   Tn(pb-CAG-cas9*,-EGFP)1Yangh involves 1 genes/genome features (Dpysl2) View all   Mutation details: The targeting vector contains a CAG promoter, floxed STOP cassette, dCas9 sequence, HA tag, 10 copies of the GCN4 (General Control Protein) epitope peptide sequence, P2A, scFv sequence encoding cognate single-chain antibody that recognizes GCN4, P65-HSF1 activator helper complex, T2A, EGFP, and a woodchuck hepatitis post transcriptional regulatory element (WPRE). This construct was flanked by piggyBac transposase inverted terminal repeats and was co-injected with a plasmid encoding piggyBac transposase expressed under the control of a beta-actin promoter. Genetic analysis at The Jackson Laboratory indicates that one copy of the transgene inserted on chromosome 14 within Dpysl2 intron 3, rather than than the Gm33925 locus as originally reported. It is not known if the transgene insertion has any effect on Dpysl2 expression. (J:259469)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Tn(pb-CAG-cas9*,-EGFP)1Yangh Mutation:	0 strains or lines available

Original:	J:259469 Zhou H, et al., In vivo simultaneous transcriptional activation of multiple genes in the brain using CRISPR-dCas9-activator transgenic mice. Nat Neurosci. 2018 Mar;21(3):440-446
All:	3 reference(s)