Casp3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:248338 Teoh JJ, et al., BIG1 is required for the survival of deep layer neurons, neuronal polarity, and the formation of axonal tracts between the thalamus and neocortex in developing brain. PLoS One. 2017;12(4):e0175888
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6149049
Gene symbol:	Casp3
Gene name:	caspase 3

Antibody:	anti-cleaved Caspase-3 (Asp175; #9661)
Detection system:	Secondary antibody coupled to Alexa Fluor 594
Assay notes:	This antibody recognizes only the cleaved form of the protein.

Results

3D ctrl E13.5 ©

Specimen 3D ctrl E13.5: embryonic day 13.5 (more

)

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Single cells	3D ctrl E13.5

3D geo/geo E13.5 ©

Specimen 3D geo/geo E13.5: embryonic day 13.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Regionally restricted	3D geo/geo E13.5	Expression is in apoptotic cells in the perirhinal and ectorhinal areas.

3D ctrl E15.5 ©

Specimen 3D ctrl E15.5: embryonic day 15.5 (more

)

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	3D ctrl E15.5

3D geo/geo E15.5 ©

Specimen 3D geo/geo E15.5: embryonic day 15.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS23: neocortex	Strong	Regionally restricted	3D geo/geo E15.5	Expression is in apoptotic cells.

3D ctrl E17.5 ©

Specimen 3D ctrl E17.5: embryonic day 17.5 (more

)

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Present	Single cells	3D ctrl E17.5

3D geo/geo E17.5 ©

Specimen 3D geo/geo E17.5: embryonic day 17.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)

Structure	Level	Pattern	Image	Note
TS25: hippocampus	Strong	Regionally restricted	3D geo/geo E17.5	Expression is in apoptotic cells.

4A ctrl Caspase-3 ©

4A ctrl Fro Caspase-3 ©

4A ctrl Fro Merged ©

4A ctrl Max Caspase-3 ©

4A ctrl Max Merged ©

4A ctrl Merged ©

Specimen 4A ctrl: embryonic day 13.5 (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Single cells	4A ctrl Fro Merged , 4A ctrl Max Caspase-3 , 4A ctrl Caspase-3 , 4A ctrl Merged , 4A ctrl Max Merged , 4A ctrl Fro Caspase-3

4A geo/geo Caspase-3 ©

4A geo/geo Fro Caspase-3 ©

4A geo/geo Fro Merged ©

4A geo/geo Max Caspase-3 ©

4A geo/geo Max Merged ©

4A geo/geo Merged ©

Specimen 4A geo/geo: embryonic day 13.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Single cells	4A geo/geo Max Merged , 4A geo/geo Caspase-3 , 4A geo/geo Max Caspase-3 , 4A geo/geo Merged , 4A geo/geo Fro Caspase-3 , 4A geo/geo Fro Merged	Expression is in an increased number of apoptotic cells at the intermediate zone/cortical plate boundary.

4B ctrl Caspase-3 ©

4B ctrl Fro Caspase-3 ©

4B ctrl Fro Merged ©

4B ctrl Max Caspase-3 ©

4B ctrl Max Merged ©

4B ctrl Merged ©

Specimen 4B ctrl: embryonic day 15.5 (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	4B ctrl Caspase-3 , 4B ctrl Max Merged , 4B ctrl Fro Caspase-3 , 4B ctrl Merged , 4B ctrl Fro Merged , 4B ctrl Max Caspase-3

4B geo/geo Caspase-3 ©

4B geo/geo Fro Caspase-3 ©

4B geo/geo Fro Merged ©

4B geo/geo Max Caspase-3 ©

4B geo/geo Max Merged ©

4B geo/geo Merged ©

Specimen 4B geo/geo: embryonic day 15.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	4B geo/geo Merged , 4B geo/geo Fro Caspase-3 , 4B geo/geo Max Merged , 4B geo/geo Max Caspase-3 , 4B geo/geo Fro Merged , 4B geo/geo Caspase-3	Expression is in an increased number of apoptotic cells at the intermediate zone/cortical plate boundary.

4C ctrl Caspase-3 ©

4C ctrl Max Caspase-3 ©

4C ctrl Max Merged ©

4C ctrl Merged ©

Specimen 4C ctrl: embryonic day 17.5 (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	4C ctrl Caspase-3 , 4C ctrl Max Merged , 4C ctrl Max Caspase-3 , 4C ctrl Merged

4C geo/geo Caspase-3 ©

4C geo/geo Max Caspase-3 ©

4C geo/geo Max Merged ©

4C geo/geo Merged ©

Specimen 4C geo/geo: embryonic day 17.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Tbr1 (assay MGI:6149050).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	4C geo/geo Caspase-3 , 4C geo/geo Max Merged , 4C geo/geo Merged , 4C geo/geo Max Caspase-3	Expression is in an increased number of apoptotic cells at the intermediate zone/cortical plate boundary.

S1A ctrl Caspase-3 ©

S1A ctrl Cor Caspase-3 ©

S1A ctrl Cor Merged ©

S1A ctrl Hip Caspase-3 ©

S1A ctrl Hip Merged ©

S1A ctrl Merged ©

Specimen S1A ctrl: embryonic day 15.5 (more

)
Note: Double labeled: red - Casp3; green - Prox1 (assay MGI:6149069).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	S1A ctrl Caspase-3 , S1A ctrl Merged , S1A ctrl Cor Caspase-3 , S1A ctrl Cor Merged
TS23: hippocampus	Present	Single cells	S1A ctrl Caspase-3 , S1A ctrl Hip Caspase-3 , S1A ctrl Merged , S1A ctrl Hip Merged

S1A geo/geo Caspase-3 ©

S1A geo/geo Cor Caspase-3 ©

S1A geo/geo Cor Merged ©

S1A geo/geo Hip Caspase-3 ©

S1A geo/geo Hip Merged ©

S1A geo/geo Merged ©

Specimen S1A geo/geo: embryonic day 15.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Prox1 (assay MGI:6149069).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	S1A geo/geo Cor Merged , S1A geo/geo Caspase-3 , S1A geo/geo Cor Caspase-3 , S1A geo/geo Merged	The number of expressing cells has increased.
TS23: hippocampus	Present	Single cells	S1A geo/geo Hip Merged , S1A geo/geo Hip Caspase-3 , S1A geo/geo Merged , S1A geo/geo Caspase-3	The number of expressing cells has increased.

S1B ctrl Caspase-3 ©

S1B ctrl Cor Caspase-3 ©

S1B ctrl Cor Merged ©

S1B ctrl Hip Caspase-3 ©

S1B ctrl Hip Merged ©

S1B ctrl Merged ©

Specimen S1B ctrl: embryonic day 17.5 (more

)
Note: Double labeled: red - Casp3; green - Calb1 (assay MGI:6149070).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	S1B ctrl Merged , S1B ctrl Cor Merged , S1B ctrl Caspase-3 , S1B ctrl Cor Caspase-3
TS25: hippocampus	Present	Single cells	S1B ctrl Caspase-3 , S1B ctrl Cor Merged , S1B ctrl Cor Caspase-3 , S1B ctrl Merged , S1B ctrl Hip Caspase-3 , S1B ctrl Hip Merged

S1B geo/geo Caspase-3 ©

S1B geo/geo Cor Caspase-3 ©

S1B geo/geo Cor Merged ©

Specimen S1B geo/geo: embryonic day 17.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Calb1 (assay MGI:6149070).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	S1B geo/geo Hip Caspase-3 , S1B geo/geo Hip Merged , S1B geo/geo Cor Caspase-3 , S1B geo/geo Merged , S1B geo/geo Cor Merged , S1B geo/geo Caspase-3	The number of expressing cells has increased.
TS25: hippocampus	Present	Single cells	S1B geo/geo Caspase-3 , S1B geo/geo Hip Caspase-3 , S1B geo/geo Merged , S1B geo/geo Hip Merged	The number of expressing cells has increased.

Specimen S2A ctrl: embryonic day 13.5 (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Single cells	S2A ctrl Caspase-3 , S2A ctrl Max Merged , S2A ctrl Merged , S2A ctrl Max Caspase-3

Specimen S2A geo/geo: embryonic day 13.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS21: neocortex	Present	Single cells	S2A geo/geo Merged , S2A geo/geo Max Caspase-3 , S2A geo/geo Max Merged , S2A geo/geo Caspase-3	The number of expressing cells has increased.

Specimen S2B ctrl: embryonic day 15.5 (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	S2B ctrl Max Merged , S2B ctrl Merged , S2B ctrl Caspase-3 , S2B ctrl Max Caspase-3

Specimen S2B geo/geo: embryonic day 15.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS23: neocortex	Present	Single cells	S2B geo/geo Max Caspase-3 , S2B geo/geo Merged , S2B geo/geo Max Merged , S2B geo/geo Caspase-3	The number of expressing cells has increased.

Specimen S2C ctrl: embryonic day 17.5 (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	S2C ctrl Merged , S2C ctrl Max Merged , S2C ctrl Caspase-3 , S2C ctrl Max Caspase-3

Specimen S2C geo/geo: embryonic day 17.5; Arfgef1^{Gt(CSH465)Byg}/Arfgef1^{Gt(CSH465)Byg} (more

)
Note: Double labeled: red - Casp3; green - Bcl11b (assay MGI:6149071).

Structure	Level	Pattern	Image	Note
TS25: neocortex	Present	Single cells	S2C geo/geo Max Merged , S2C geo/geo Caspase-3 , S2C geo/geo Merged , S2C geo/geo Max Caspase-3	The number of expressing cells has increased.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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