Probe:	Dmbx1 probe4
Probe preparation:	labelled with digoxigenin RNA
Assay notes:	Authors report using two different probes, which gave identical results. Dmbx1 cDNA4 (MGI:6121414) may also have been used.

Structure	Level	Pattern	Image	Note
TS11: future brain	Present	Regionally restricted	3A	Expression around prospective midbrain region.

Structure	Level	Pattern	Image	Note
TS12: future midbrain	Present		3B	More definite expression, limited to prospective midbrain region exclusively.

Structure	Level	Pattern	Image	Note
TS14: future midbrain	Present		3C	Expanded domain of midbrain expression.
TS14: optic eminence	Present		3C	Expression extending partly into optic eminence.

Structure	Level	Pattern	Image	Note
TS16: diencephalon	Present		3D	Expression rostrally in diencephalon.
TS16: future midbrain	Present	Widespread	3D	Clearest site of expression in midbrain, with expression all across the developing structure.
TS16: nasal pit	Present	Regionally restricted	3D	Clear expression in medial and lateral nasal pits.
TS16: optic cup	Present	Regionally restricted	3D	Clear expression in dorsal half of optic cup.
TS16: future hindbrain	Present	Regionally restricted	3D	Clear expression in parts of hindbrain.

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Regionally restricted	3E	Hindbrain expression resolved into 2 anteroposteriorly oriented stripes along lateral edges of rhombomeres, on each side of midline.
TS18: forelimb bud	Present	Regionally restricted	3E	Small but distinct region of expression in posterior, distal region of forelimb bud.

Structure	Level	Pattern	Image	Note
TS19: diencephalon	Present		3F
TS19: hindbrain	Present		3F
TS19: midbrain	Present		3F
TS19: midbrain-hindbrain junction	Absent		3F	Expression absent directly at site of mibrain-hindbrain boundary.
TS19: nose	Present	Regionally restricted	3F	Nasal expression on either side of naso-lacrimal groove.
TS19: eye	Weak	Regionally restricted	3F	Weakened expression in optic cup.

Structure	Level	Pattern	Image	Note
TS18: forelimb bud	Present	Regionally restricted	3G	Small but distinct region of expression in posterior, distal region of forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Absent		3H

Structure	Level	Pattern	Image	Note
TS19: forelimb bud	Absent		3I

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	3J	Expression along distal edge of hindlimb bud, subjacent to apical ectodermal ridge.

Structure	Level	Pattern	Image	Note
TS18: hindbrain	Present	Regionally restricted	3K	Expression in two parallel, bilateral stripes of cells running longitudinally.
TS18: rhombomere	Present	Regionally restricted	3K	The more medial stripe runs from rhombomere 2 to the spinal cord.

Structure	Level	Pattern	Image	Note
TS18: rhombomere 5	Present	Regionally restricted	3L	The more lateral stripe is more caudal, limited to rhombomeres 5 and 6.
TS18: rhombomere 6	Present	Regionally restricted	3L	The more lateral stripe is more caudal, limited to rhombomeres 5 and 6.