Gene Expression Data
Assay Details
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Assay
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Reference:
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J:239322
Sailau ZK, et al., Nuclear distribution of the chromatin-remodeling protein ATRX in mouse early embryogenesis. Acta Histochem. 2017 Jan;119(1):18-25
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6117769
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Gene symbol:
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Atrx
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Gene name:
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ATRX, chromatin remodeler
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Results
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Specimen
1:
embryonic day 0.5
(more  )
Note: Early zygote stage (20 h post hGG).
Specimen
1:
(close  )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early zygote stage (20 h post hGG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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1
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Not expressed in the nucleolus precursor bodies. The rest of the nucleoplasm is stained diffusely.
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Specimen
2:
embryonic day 0.5
(more )
Note: Late zygote stage (27 h post hGG).
Specimen
2:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late zygote stage (27 h post hGG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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2
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Positively stained ring-shaped or arcuate zones, which partially coincide with the DAPI-positive areas are revealed in the close vicinity to the nucleolus precursor body periphery.
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Specimen
3 left:
embryonic day 0.5
(more )
Note: Late zygote stage (27 h post hGG).
Specimen
3 left:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late zygote stage (27 h post hGG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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3_left
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A weak staining along the entire nucleolus precursor body periphery.
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Specimen
3 middle:
embryonic day 0.5
(more )
Note: Late zygote stage (27 h post hGG).
Specimen
3 middle:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late zygote stage (27 h post hGG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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3_middle
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Staining of individual zones in the nucleolus precursor bodies.
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Specimen
3 right:
embryonic day 0.5
(more )
Note: Late zygote stage (27 h post hGG).
Specimen
3 right:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 0.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late zygote stage (27 h post hGG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS1: 1-cell stage embryo |
Present |
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3_right
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More intense fluorescence of several regions at the nucleolus precursor body periphery.
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Specimen
4:
embryonic day 1.0
(more )
Note: Early 2-cell stage (32 h post hCG).
Specimen
4:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Early 2-cell stage (32 h post hCG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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4
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Intense staining were observed at the periphery of the nucleolus precursor bodies, together with a diffuse staining of the nucleoplasm. A colocalization of heterochromatin and ATRX was detected in some areas at the nucleolus precursor body periphery. The presence of heterochromatin areas that do not contain ATRX is clearly seen.
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Specimen
5:
embryonic day 1.0
(more )
Note: Late 2-cell stage (46 h post hCG).
Specimen
5:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Late 2-cell stage (46 h post hCG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS2: 2-cell stage embryo |
Present |
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5
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Intense staining in the structures of roundish shape that are more often located in the nucleoplasm outside the nucleolus precursor body (NPBs), and in elongated arcuate shape zones localized at the periphery of the NPBs. The rest of the nucleoplasm is stained diffusely. Colocalized with heterochromatin in fewareas at the NPB periphery as well as in the nucleoplasm without association with NPBs.
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Specimen
6:
embryonic day 2.0
(more )
Note: 4-cell stage (55 h post hCG).
Specimen
6:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 2.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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4-cell stage (55 h post hCG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS3: 4-cell stage embryo |
Present |
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6
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Intense staining in heterochromatin areas located either in the vicinity of the nucleolus precursor body (NPB) periphery or randomly distributed in the nucleoplasm without evident association with NPBs.
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Specimen
7:
embryonic day 3.0
(more )
Note: Morula stage (72 h post hCG).
Specimen
7:
(close )
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Genetic Background:
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BALB/c
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Age:
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embryonic day 3.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Morula stage (72 h post hCG).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS4: compacted morula |
Present |
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7
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No intense staining at the periphery of the developing nucleoli. Rarely colocalized with heterochromatin. Expressed in the nucleoplasm and rarely at the nucleolar periphery.
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Analysis Tools