Gene Expression Data
Assay Details
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Assay
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Reference:
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J:126306
Nural HF, et al., The Slit receptor Robo1 is predominantly expressed via the Dutt1 alternative promoter in pioneer neurons in the embryonic mouse brain and spinal cord. Gene Expr Patterns. 2007 Oct;7(8):837-45
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5927616
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Gene symbol:
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Robo1
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Gene name:
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roundabout guidance receptor 1
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Probe:
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Robo1 cDNA8
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2C:
embryonic day 10.5
(more  )
Specimen
2C:
(close  )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon |
Weak |
Regionally restricted |
2C
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Expression extends in patches from the base of the optic stalk through ventral thalamus.
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| TS17: optic stalk |
Weak |
Regionally restricted |
2C
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Expression extends in patches from the base of the optic stalk through ventral thalamus.
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| TS17: midbrain |
Present |
Patchy |
2C
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Expression is in the anterior midbrain.
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| TS17: hindbrain |
Present |
Regionally restricted |
2C
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Expression is in patches in the anterior hindbrain.
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| TS17: hindbrain lateral wall |
Present |
Regionally restricted |
2C
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Expression is in a band at the level of the motor columns.
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Specimen
3C:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
3C:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: diencephalon mantle layer |
Present |
Regionally restricted |
3D
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3C
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Expression is in neurons in the mantle layer of the ventral thalamus and is absent in the neuroepithelial layer.
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Specimen
4E:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
4E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: hindbrain |
Present |
Regionally restricted |
4E
,
4G
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4F
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Expression is restricted to a small subset within dorsal and ventral neurons.
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| TS17: hindbrain lateral wall |
Present |
Regionally restricted |
4F
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4E
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4G
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Expression is laterally in the area of the hindbrain motor neurons.
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Specimen
5E:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
5E:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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Specimen
7D:
embryonic day 10.5
(more )
Note: Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
Specimen
7D:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Robo1; brown - immunohistochemistry with Tubb3 (assay MGI:5927618).
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Specimen
not shown:
embryonic day 10.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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CD-1
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: midbrain |
Present |
Single cells |
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Expression is in a few scattered cells.
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Analysis Tools