Probe:	Ebf3 cDNA2
Probe preparation:	Antisense labelled with S35 RNA

Structure	Level	Pattern	Image	Note
TS18: neural tube mantle layer	Present		3G
TS18: neural tube basal columns	Weak		3G

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Present	Regionally restricted	4F	Expression is caudal.
TS19: hindbrain ventricular layer	Present	Regionally restricted	4F	Expression is in the rostral-most hindbrain.
TS19: midbrain-hindbrain junction	Absent		4F

Structure	Level	Pattern	Image	Note
TS19: hindbrain mantle layer	Present		4J
TS19: hindbrain ventricular layer	Present	Single cells	4J	Expression is in a few cells.

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted	4M	Expression is in the vestibular nuclei and superior olive.
TS22: cerebellum gray matter	Present	Regionally restricted	4M	Expression is in the cortical transitory zone.
TS22: deep cerebellar nucleus	Strong		4M

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Widespread	5C
TS21: forebrain-midbrain boundary region	Strong	Regionally restricted	5C	Expression is in the precommissural zone.
TS21: thalamus	Present	Regionally restricted	5C	Expression is in the dorsal thalamus and is absent in the ventral thalamus.
TS21: posterior commissure	Present		5C
TS21: cerebral cortex	Present	Regionally restricted	5C	Expression is in the external layer.

Structure	Level	Pattern	Image	Note
TS21: mammillary area	Present		5F
TS21: zona limitans intrathalamica	Present		5F
TS21: forebrain	Present	Regionally restricted	5F	Expression is in the eminentia thalami.
TS21: cerebral cortex	Weak	Regionally restricted	5F	Expression is in the external layer.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		6B
TS20: posterior commissure	Present		6B
TS20: forebrain-midbrain boundary region	Present	Regionally restricted	6B	Expression is in the pretectum.
TS20: thalamus	Present	Regionally restricted	6B	Expression is in the ventral thalamus.
TS20: zona limitans intrathalamica	Strong		6B
TS20: forebrain	Strong	Regionally restricted	6B	Expression is in the eminentia thalami.
TS20: telencephalon	Present	Regionally restricted	6B	Expression is in a thin external layer of the septum.

Structure	Level	Pattern	Image	Note
TS20: hypothalamus	Present	Regionally restricted	6I	Expression is absent in a longitudinal band at the level of the hypothalamic cell cord.

Structure	Level	Pattern	Image	Note
TS15: future midbrain lateral wall	Present	Single cells		Expression is in a few cells in the alar plate.
TS15: diencephalon	Present	Regionally restricted		Expression is in the alar and basal plates of the caudal part of the diencephalon.

Structure	Level	Pattern	Image	Note
TS16: future midbrain	Present	Graded		There is a rostrocaudal decreasing gradient of expression.

Structure	Level	Pattern	Image	Note
TS21: midbrain	Present	Regionally restricted		From E13.5 there is a progressive down-regulation of expression in the basal plate, while expression was maintained in the tectum.
TS21: main olfactory bulb	Present
TS21: retina	Present

Structure	Level	Pattern	Image	Note
TS22: pons	Present	Regionally restricted		Expression is in the cochlear nucleus.
TS22: corpus striatum	Present
TS22: hippocampus	Present
TS22: pretectum	Present
TS22: cerebral cortex	Present	Regionally restricted		Expression is in the external layer.
TS22: midbrain	Present	Regionally restricted		Expression is downregulated in the basal plate.