Gene Expression Data
Assay Details
Assay
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Reference:
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J:43984
Garel S, et al., Family of Ebf/Olf-1-related genes potentially involved in neuronal differentiation and regional specification in the central nervous system. Dev Dyn. 1997 Nov;210(3):191-205
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:5912308
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Gene symbol:
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Ebf1
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Gene name:
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early B cell factor 1
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Probe:
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Ebf cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Results
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Specimen
3A:
embryonic day 10.0
(more )
Specimen
3A:
(close )
Genetic Background:
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CRL:OF1
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Age:
|
embryonic day 10.0
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Sex:
|
Not Specified
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Type:
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section from whole mount
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
4A:
embryonic day 9.5
(more )
Note: Flat mount.
Specimen
4A:
(close )
Genetic Background:
|
CRL:OF1
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Age:
|
embryonic day 9.5
|
Sex:
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Not Specified
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Type:
|
whole mount
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Fixation:
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4% Paraformaldehyde
|
Note:
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Flat mount.
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: rhombomere 2 |
Strong |
Regionally restricted |
4A
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Expression is in the basal plate.
|
TS15: rhombomere 4 |
Strong |
Regionally restricted |
4A
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Expression is in the basal plate.
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TS15: rhombomere 3 |
Present |
Regionally restricted |
4A
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Expression is in the ventromedial part of the r2/r3 boundary.
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TS15: rhombomere 5 |
Present |
Regionally restricted |
4A
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Expression is in the ventromedial part of the r4/r5 boundary.
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Specimen
4B:
embryonic day 10.0
(more )
Specimen
4B:
(close )
Genetic Background:
|
CRL:OF1
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Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
4C:
embryonic day 10.5
(more )
Note: Flat mount.
Specimen
4C:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 10.5
|
Sex:
|
Not Specified
|
Type:
|
whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Note:
|
Flat mount.
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: hindbrain |
Present |
Regionally restricted |
4C
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Expression is in the ventromedial region and in a columnar pattern in the dorsolateral region.
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TS17: rhombomere |
Present |
Regionally restricted |
4C
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Expression is stronger at the inter-rhombomeric boundaries.
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TS17: hindbrain mantle layer |
Present |
Regionally restricted |
4C
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Expression is at the basal mantle layer.
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Specimen
not shown E9.5:
embryonic day 9.5
(more )
Specimen
not shown E9.5:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 9.5
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Structure |
Level |
Pattern |
Image |
Note |
TS15: neural tube lateral wall |
Present |
Single cells |
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Expression is in the ventrolateral part in a few cells likely to correspond to motor neurons.
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Specimen
not shown E9.0:
embryonic day 9.0
(more )
Specimen
not shown E9.0:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 9.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Specimen
not shown E10.0:
embryonic day 10.0
(more )
Specimen
not shown E10.0:
(close )
Genetic Background:
|
CRL:OF1
|
Age:
|
embryonic day 10.0
|
Sex:
|
Not Specified
|
Type:
|
section from whole mount
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Gelatin
|
Structure |
Level |
Pattern |
Image |
Note |
TS16: rhombomere lateral wall |
Present |
Regionally restricted |
|
Expression is in the motor columns of the odd-numbered rhombomeres.
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