Antibody:	anti-APP
Detection system:	Secondary antibody coupled to alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	1A	Expressed in the mantle layer of the ventral hindbrain, concentrated close to the midline as a cluster that extended laterally to form a layer.
TS15: trigeminal placode	Present		1A

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		1B
TS15: neural tube lateral wall	Present	Regionally restricted	1B	Expressed in the ventral-lateral area of the spinal cord and the future ventral horn. Only a few positive cells in the ventral-lateral intermediate zone. No expression in the ventricular zone.

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	1D	Expressed in the mantle layer cells of the ventral part. Concentrated close to the midline in an area that will give rise to the trigeminal nucleus.
TS17: trigeminal ganglion	Present		1D

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	1E	Expressed in the lateral part.
TS17: acoustic ganglion	Present		1E
TS17: facial ganglion	Present		1E

Structure	Level	Pattern	Image	Note
TS17: hindbrain	Present	Regionally restricted	1F	Expressed in the ventral and lateral areas, and the cranial nerve rootlets.
TS17: vagus ganglion	Present		1F

Structure	Level	Pattern	Image	Note
TS17: future spinal cord	Present		1G
TS17: neural tube floor plate	Present		1G
TS17: spinal nerve	Present		1G
TS17: sympathetic nerve trunk	Present		1G
TS17: dorsal root ganglion	Weak		1G

Structure	Level	Pattern	Image	Note
TS17: neural tube floor plate	Present		1H
TS17: neural tube lateral wall	Present	Regionally restricted	1H	Expressed in the ventral horn.

Structure	Level	Pattern	Image	Note
TS17: sympathetic nerve trunk	Present		1I

Structure	Level	Pattern	Image	Note
TS20: facial ganglion	Present		2A
TS20: trigeminal ganglion	Present		2A

Structure	Level	Pattern	Image	Note
TS20: glossopharyngeal ganglion	Present		2B
TS20: vagus ganglion	Present		2B

Structure	Level	Pattern	Image	Note
TS20: spinal cord lateral wall	Present	Regionally restricted	2C	Expressed in the intermediate zone. Increase number of expressed cells, particularly in the ventral-lateral quadrant, the future motor horn.
TS20: dorsal root ganglion	Present		2C
TS20: spinal cord floor plate	Present		2C
TS20: spinal cord ventricular layer	Absent		2C

Structure	Level	Pattern	Image	Note
TS20: olfactory epithelium	Present		2D

Structure	Level	Pattern	Image	Note
TS21: trigeminal ganglion	Present		2E

Structure	Level	Pattern	Image	Note
TS21: vagus ganglion	Present		2F	Expressed in cells of the dorsal motor nucleus of the vagus nerve.

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Present	Regionally restricted	2G	Expressed in cells of the nucleus of the hypoglossal nerve.

Structure	Level	Pattern	Image	Note
TS21: spinal cord lateral wall	Strong	Regionally restricted	2H	Expressed in the motor columns of the ventral horn. Decrease number of expressed cells in the ventral-lateral quadrant, the future motor horn.
TS21: dorsal root ganglion	Present		2H
TS21: spinal cord floor plate	Present		2H
TS21: spinal cord ventricular layer	Absent		2H

Structure	Level	Pattern	Image	Note
TS21: parasympathetic ganglion	Present	Regionally restricted	2I	Expressed in the ciliary ganglion.
TS21: olfactory epithelium	Weak		2I

Structure	Level	Pattern	Image	Note
TS19: myelencephalon basal plate	Weak	Regionally restricted	3D	Expressed in the intermediate zone.
TS19: myelencephalon	Strong		3D	A much stronger expression in neurons of the hypoglossal and vagus nuclei.

Structure	Level	Pattern	Image	Note
TS19: myelencephalon	Present	Regionally restricted	3F	Stronger expression in the dorsal motor nucleus of the vagus (X) nerve, and neurons of the nucleus of hypoglossal (XII) nerve, compared with the cells in the surrounding tissue.
TS19: myelencephalon floor plate	Present		3F

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Strong	Regionally restricted	3G	Expressed in neurons of the nucleus of hypoglossal nerve, and in the dorsal motor nucleus of vagus nerve.

Structure	Level	Pattern	Image	Note
TS21: metencephalon	Present	Regionally restricted	3H	Expressed in the neurons of the motor nucleus.
TS21: trigeminal ganglion	Present		3H

Structure	Level	Pattern	Image	Note
TS21: telencephalon	Weak	Regionally restricted	3I	Expressed in the ventral-lateral aspect of the posterior part.
TS21: trigeminal ganglion	Present		3I
TS21: hypothalamus	Weak		3I

Structure	Level	Pattern	Image	Note
TS13: embryo	Absent

Structure	Level	Pattern	Image	Note
TS15: future midbrain	Present
TS15: future forebrain	Absent
TS15: geniculate placode	Present
TS15: nodose placode	Present
TS15: petrosal placode	Present
TS15: otocyst epithelium	Present			Exprssed in the cells of the inner surface of the otic vesicle.

Structure	Level	Pattern	Image	Note
TS17: telencephalon	Weak	Regionally restricted		Expressed in the ventrallateral area.
TS17: Rathke's pouch	Weak
TS17: glossopharyngeal ganglion	Present

Structure	Level	Pattern	Image	Note
TS19: cranial ganglion	Present
TS19: nasal pit	Present
TS19: midbrain mantle layer	Present	Regionally restricted		Expressed a single layer of cells in the mantle layer.

Structure	Level	Pattern	Image	Note
TS20: hindbrain	Present	Clusters		Expressed in the clustered cells in the neural tube, which begin to form the motor nuclei of the cranial nerves.
TS20: sympathetic ganglion	Present

Structure	Level	Pattern	Image	Note
TS21: medulla oblongata	Weak	Regionally restricted		A cluster of cells in the myelencephalon located ventrally to the hypoglossal nucleus were weakly labeled and are presumably cells of the nucleus ambiguus.
TS21: lung	Weak
TS21: sympathetic ganglion	Present