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Gene Expression Data
Assay Details
Assay
Reference: J:18811 Sheppard AM, et al., Expanding roles for alpha 4 integrin and its ligands in development. Cell Adhes Commun. 1994 Apr;2(1):27-43
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5911016
Gene symbol: Vcam1
Gene name: vascular cell adhesion molecule 1
Antibody: M/K-1
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Either this antibody oo anti-NCAM-110 (MGI:5911487) was used.
Results
Specimen 1d': embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: back musculature Present 1d'
Specimen 2e: embryonic day 15.5 (more )
Structure Level Pattern Image Note
TS23: lung mesenchyme Present 2e
TS23: lung vascular element Absent 2e
Specimen 3b: postnatal adult (more )
Structure Level Pattern Image Note
TS28: intestinal villus Very strong Scattered 3b Expression is in mononuclear cells scattered throughout the villi.
TS28: small intestine smooth muscle Absent 3b
Specimen 3f: postnatal adult (more )
Structure Level Pattern Image Note
TS28: stomach smooth muscle Present Punctate 3f
TS28: stomach Strong Regionally restricted 3f Expression is in single cells in the adventitia.
Specimen 4b: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: atrioventricular endocardial cushion Present 4b
TS21: atrioventricular valve Present 4b
TS21: interventricular septum Present 4b
TS21: dorsal aorta Weak 4b
Specimen 5b: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: liver Present Single cells 5b The authors state that expression is in cells likely to be macrophages.
Specimen 5d: postnatal adult (more )
Note: Double labeled: green - Vcam1; red - Fn1 (assay MGI:5911109).
Structure Level Pattern Image Note
TS28: spleen marginal zone Present Regionally restricted 5d' , 5d Expression is between the nodules and the red pulp.
TS28: spleen white pulp Weak 5d' , 5d
Specimen 5e: postnatal adult (more )
Note: Double labeled: green - Vcam1; red - Fn1 (assay MGI:5911109).
Structure Level Pattern Image Note
TS28: spleen marginal zone Present Regionally restricted 5e , 5e' Expression is between the nodules and the red pulp.
TS28: spleen white pulp Weak 5e' , 5e
Specimen 5g: postnatal adult (more )
Structure Level Pattern Image Note
TS28: thymus medullary core Present 5g
TS28: thymus cortex Weak 5g
Specimen 6b: postnatal adult (more )
Note: Double labeled: green - Vcam1; red - Fn1 (assay MGI:5911109).
Structure Level Pattern Image Note
TS28: renal small blood vessel Present Regionally restricted 6b , 6b'
TS28: renal tubule epithelium Absent 6b , 6b'
Specimen 8e: postnatal adult (more )
Structure Level Pattern Image Note
TS28: blood vessel endothelium Very strong 8e
TS28: myometrium Absent 8e
Specimen 9d: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: yolk sac mesenchyme Present Regionally restricted 9d Expression is in the subset of the blood islands in vessels with large lumens on the interior side.
Specimen not shown E9: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: dorsal root ganglion Absent
TS15: embryo ectoderm Absent
Specimen not shown E13: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: eye Absent
Specimen not shown: postnatal adult (more )
Structure Level Pattern Image Note
TS28: heart Absent
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/30/2024
MGI 6.23 		The Jackson Laboratory