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Gene Expression Data
Assay Details
Assay
Reference: J:239876 Xu Q, et al., Maternal BCAS2 protects genomic integrity in mouse early embryonic development. Development. 2015 Nov 15;142(22):3943-53
Assay type: Immunohistochemistry
MGI Accession ID: MGI:5910517
Gene symbol: Chek1
Gene name: checkpoint kinase 1
Antibody: anti-pCHK1 (S345)
Detection system: Secondary antibody
Assay notes: The antibody recognizes the phosphorylated form of the protein.
Results
Specimen 5G Control: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2+, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: green - Chek1; red - H2ax (assay MGI:5910436).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Trace 5G Control Merge , 5G Control CHK1(p345S)
Specimen 5G: embryonic day 1.0; Bcas2tm1.1Lil/Bcas2tm1.1Lil, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. 2-cell embryo 48 h post-hCG. Double labeled: green - Chek1; red - H2ax (assay MGI:5910436).
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present 5G Bcas2mNull Chk1(p345S) , 5G Bcas2mNull Merge
Specimen S4B 37: embryonic day 1.0 (more )
Note: 2-cell embryo 48 h post-hCG. Double labeled: green - Chek1; red - H2ax (assay MGI:5910436). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Present S4B Merge 37 , S4B CHK1(p345S) 37
Specimen S4B 48: embryonic day 1.0 (more )
Note: 2-cell embryo 48 h post-hCG. Double labeled: green - Chek1; red - H2ax (assay MGI:5910436). Specimens were treated with Triton for 1 minute prior to fixation.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak S4B CHK1(p345S) 48 , S4B Merge 48
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/16/2024
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