Probe:

Slitrk6 probe2

Structure	Level	Pattern	Image	Note
TS13: head	Present	Regionally restricted	1A	Expression in head folds of midbrain region.
TS13: otic placode epithelium	Present		1A	Expression in otocyst-forming ectoderm.
TS13: trunk surface ectoderm	Present	Regionally restricted	1A	Expression in lateral end of epidermal ectoderm.

Structure	Level	Pattern	Image	Note
TS13: future midbrain	Present	Regionally restricted	1B	Expression limited to head folds of midbrain region.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Strong		1C
TS15: surface ectoderm	Strong	Regionally restricted	1C	Stronger expression in lateral epidermis.

Structure	Level	Pattern	Image	Note
TS15: otocyst	Strong	Regionally restricted	1D	Especially strong expression in rostro-lateral part of otocyst.

Structure	Level	Pattern	Image	Note
TS17: branchial arch	Present	Regionally restricted	1E
TS17: 1st branchial arch	Strong		1E
TS17: otocyst	Weak		1E
TS17: surface ectoderm	Present	Regionally restricted	1E	Expression in lateral epidermis.
TS17: forelimb bud ectoderm	Present	Regionally restricted	1E	Expression continues into proximal limb buds.
TS17: hindlimb bud ectoderm	Present	Regionally restricted	1E	Expression continues into proximal limb buds.

Structure	Level	Pattern	Image	Note
TS17: surface ectoderm	Present	Regionally restricted	1F	Expression in lateral epidermis.
TS17: forelimb bud ectoderm	Present	Regionally restricted	1F	Expression continues into proximal limb buds.
TS17: hindlimb bud ectoderm	Present	Regionally restricted	1F	Expression continues into proximal limb buds.
TS17: spinal nerve	Weak	Regionally restricted	1F	Faint expression in the form of a thin line along the anterior-posterior axis, corresponding to dorsal branches of spinal nerves.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Strong	Regionally restricted	1G top	Stronger expression in dorsal aspect than in ventral aspect.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	1G bottom

Structure	Level	Pattern	Image	Note
TS19: forelimb bud ectoderm	Present	Regionally restricted	1H	Expression limited to epidermis and underlying mesenchymal cells located at junction of limb bud and trunk.
TS19: forelimb bud mesenchyme	Present	Regionally restricted	1H	Expression limited to epidermis and underlying mesenchymal cells located at junction of limb bud and trunk.
TS19: lung rudiment	Present		1H	Expression in lung bud.

Structure	Level	Pattern	Image	Note
TS20: esophagus	Present	Regionally restricted	1I	Strong expression in visceral organs. Authors report expression in two layers.
TS20: lung	Present	Regionally restricted	1I	Strong expression in visceral organs. Authors report expression in two layers.
TS20: midgut	Present	Regionally restricted	1I	Strong expression in visceral organs. Authors report expression in two layers.
TS20: stomach	Present	Regionally restricted	1I	Strong expression in visceral organs. Authors report expression in two layers.
TS20: liver	Absent		1I

Structure	Level	Pattern	Image	Note
TS19: lung rudiment mesenchyme	Present	Regionally restricted	1J	Expression in lateral part of lung bud (distal end of parenchyma) and mesenchyme surrounding main bronchi.
TS19: esophagus	Present		1J

Structure	Level	Pattern	Image	Note
TS19: midgut	Present	Regionally restricted	1K	Expression in subepithelial layer of midgut.
TS19: enteric nervous system	Present	Regionally restricted	1K	Expression in enteric neurons.

Structure	Level	Pattern	Image	Note
TS22: pancreas	Present

Structure	Level	Pattern	Image	Note
TS20: thalamus	Absent		2A

Structure	Level	Pattern	Image	Note
TS21: thalamus	Present	Regionally restricted	2C	Expression in dorsal thalamus. No expression in ventrolateral part.

Structure	Level	Pattern	Image	Note
TS23: dorsal thalamus	Present		2E

Structure	Level	Pattern	Image	Note
TS25: posterior thalamic nucleus	Present		2G
TS25: ventral lateral geniculate nucleus	Present		2G
TS25: ventral posterior lateral thalamic nucleus	Present		2G

Structure	Level	Pattern	Image	Note
TS25: dorsal thalamus	Present	Regionally restricted	2I

Structure	Level	Pattern	Image	Note
TS28: thalamic nucleus	Present	Regionally restricted	2K	Expression in intermediodorsal thalamic nucleus.
TS28: lateral geniculate nucleus	Present		2K

Structure	Level	Pattern	Image	Note
TS21: cerebellum ventricular layer	Present	Regionally restricted	3A	Expression in part of ventricular layer of cerebellar primordia.
TS21: pons ventricular layer	Present	Regionally restricted	3A	Expression in superficial layer of pontine neuroepithelium (ventricular layer).
TS21: medulla oblongata	Present		3A

Structure	Level	Pattern	Image	Note
TS23: cerebellum	Present	Regionally restricted	3C	Expession confined to caudal compartment. Expression densely distributed in deeper layer where Purkinje cell precursors are known to be located.
TS23: cerebellum external granule cell layer	Absent		3C

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Regionally restricted	4A	Expression in hair-cell generation region (primitive sensory neuroepithelium) of cochlear duct.
TS21: face skin	Strong	Regionally restricted	4A	Strong epidermal and dermal expression in maxillary and mandibular regions.
TS21: face mesenchyme	Present	Regionally restricted	4A	Broad expression in mesenchymal tissues constituting maxillary and mandibular structures.

Structure	Level	Pattern	Image	Note
TS21: cochlear duct epithelium	Present	Regionally restricted	4C	Expression in hair-cell generation region (primitive sensory neuroepithelium) of cochlear duct.
TS21: perioptic mesenchyme	Present		4C
TS21: vibrissa dermal component	Present	Regionally restricted	4C	Expression in dermal layer surrounding outer hair root sheath of vibrissa.

Structure	Level	Pattern	Image	Note
TS23: cochlear duct epithelium	Present	Regionally restricted	4D	Expression in primitive sensory neuroepithelium that later develops into cochlear hair cells.

Structure	Level	Pattern	Image	Note
TS23: cochlear ganglion	Present	Regionally restricted	4E	Expression in lateral edge of spiral ganglion.

Structure	Level	Pattern	Image	Note
TS23: retina nuclear layer	Present	Regionally restricted	4G	Expression in retinal outer neuroblastic layer.
TS23: eyelid	Present		4G
TS23: perioptic mesenchyme	Present		4G
TS23: face skin	Present	Regionally restricted	4G	Expression in eyelids continued into epidermal and dermal layers in skin of head surrounding eyelids.

Structure	Level	Pattern	Image	Note
TS23: vibrissa dermal component	Present	Regionally restricted	4I	Expression in dermal layer surrounding outer hair root sheath of vibrissa.

Structure	Level	Pattern	Image	Note
TS21: oral region	Present		4K
TS21: face skin	Strong	Regionally restricted	4K	Strong epidermal and dermal expression in maxillary and mandibular regions.
TS21: face mesenchyme	Present	Regionally restricted	4K	Broad expression in mesenchymal tissues constituting maxillary and mandibular structures.
TS21: nasal mesenchyme	Present	Regionally restricted	4K	Expression in mesenchymal cells surrounding posterior part of nasal epithelium.

Structure	Level	Pattern	Image	Note
TS21: face skin	Present	Regionally restricted	4M	Strong epidermal and dermal expression in maxillary and mandibular regions.
TS21: face mesenchyme	Present	Regionally restricted	4M	Broad expression in mesenchymal tissues constituting maxillary and mandibular structures.
TS21: tongue epithelium	Present	Regionally restricted	4M	Expression in upper surface of tongue.
TS21: upper jaw molar epithelium	Present	Regionally restricted	4M	Expression in invaginating oral epithelium forming upper molar teeth primordia.