Gene Expression Data
Assay Details
Assay
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Reference:
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J:86960
Darling DS, et al., Expression of Zfhep/deltaEF1 protein in palate, neural progenitors, and differentiated neurons. Gene Expr Patterns. 2003 Dec;3(6):709-17
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:5905162
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Gene symbol:
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Zeb1
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Gene name:
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zinc finger E-box binding homeobox 1
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Antibody:
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anti-Zfhep
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
5A:
embryonic day 12.5
(more )
Specimen
5A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: hindbrain |
Present |
Regionally restricted |
5A
|
Authors describe expression in a cluster of neurons.
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Specimen
5D/F:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
5D/F:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5F
,
5D
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Expression in trigeminal nucleus (Vn) of pons.
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Specimen
5G/I:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
5G/I:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Specimen
5J:
embryonic day 12.5
(more )
Specimen
5J:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5J
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Expression in cells migrating out of restricted region of basal plate of pons.
|
Specimen
5K:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
5K:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5K
|
Expression in stream of cells from restricted region of basal plate at lateral edge of pons.
|
Specimen
5L:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
5L:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5L
|
Expression in migrating cells as they differentiate.
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Specimen
5M/O:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
5M/O:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: pons |
Present |
Regionally restricted |
5M
,
5O
|
Expression in trigeminal motor nucleus.
|
Specimen
6A:
embryonic day 11.5
(more )
Specimen
6A:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Specimen
6B/D:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Brn3a (Pou4f1; assay MGI:5905170).
Specimen
6B/D:
(close )
Genetic Background:
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Not Specified
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Brn3a (Pou4f1; assay MGI:5905170).
|
Specimen
6E/H:
embryonic day 12.5
(more )
Note: Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
Specimen
6E/H:
(close )
Genetic Background:
|
Not Specified
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Age:
|
embryonic day 12.5
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Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Zeb1; red - Isl1 (assay MGI:5905169).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: cranial ganglion |
Present |
Regionally restricted |
6H
,
6E
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Expression in X and XI ganglia complex.
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